SWATH-MS: Data Acquisition and Analysis

K. Frederick, P. Ciborowski

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is an emerging proteomic platform for label free quantification. This platform is based on data independent acquisition (DIA) principle for simultaneous protein identification and quantification. SWATH-MS requires establishment of spectral library which is discussed in this chapter. SWATH-MS data analysis requires targeted data extraction, data normalization. Typical presentation of results from experiments using SWATH-MS is principal component analysis. Several pipelines of DIA analysis are available, however MarkerView and Skyline are two the most commonly used.

Original languageEnglish (US)
Title of host publicationProteomic Profiling and Analytical Chemistry
Subtitle of host publicationThe Crossroads: Second Edition
PublisherElsevier Inc.
Pages161-173
Number of pages13
ISBN (Print)9780444636881
DOIs
StatePublished - Mar 22 2016

Keywords

  • Data dependent acquisition
  • Data independent acquisition
  • MarkerView
  • Principal component analysis
  • SWATH-MS
  • Skyline
  • Statistical analysis

ASJC Scopus subject areas

  • Chemistry(all)

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