SWATH-MS: Data Acquisition and Analysis

K. Frederick; P. Ciborowski

doi:10.1016/B978-0-444-63688-1.00009-4

SWATH-MS: Data Acquisition and Analysis

K. Frederick, P. Ciborowski

Research output: Chapter in Book/Report/Conference proceeding › Chapter

9 Scopus citations

Abstract

Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is an emerging proteomic platform for label free quantification. This platform is based on data independent acquisition (DIA) principle for simultaneous protein identification and quantification. SWATH-MS requires establishment of spectral library which is discussed in this chapter. SWATH-MS data analysis requires targeted data extraction, data normalization. Typical presentation of results from experiments using SWATH-MS is principal component analysis. Several pipelines of DIA analysis are available, however MarkerView and Skyline are two the most commonly used.

Original language	English (US)
Title of host publication	Proteomic Profiling and Analytical Chemistry
Subtitle of host publication	The Crossroads: Second Edition
Publisher	Elsevier Inc.
Pages	161-173
Number of pages	13
ISBN (Print)	9780444636881
DOIs	https://doi.org/10.1016/B978-0-444-63688-1.00009-4
State	Published - Mar 22 2016

Keywords

Data dependent acquisition
Data independent acquisition
MarkerView
Principal component analysis
SWATH-MS
Skyline
Statistical analysis

ASJC Scopus subject areas

Chemistry(all)

Access to Document

10.1016/B978-0-444-63688-1.00009-4

Cite this

@inbook{c0d6c1258bc64a72a3ffd8b3bda8e687,

title = "SWATH-MS: Data Acquisition and Analysis",

abstract = "Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is an emerging proteomic platform for label free quantification. This platform is based on data independent acquisition (DIA) principle for simultaneous protein identification and quantification. SWATH-MS requires establishment of spectral library which is discussed in this chapter. SWATH-MS data analysis requires targeted data extraction, data normalization. Typical presentation of results from experiments using SWATH-MS is principal component analysis. Several pipelines of DIA analysis are available, however MarkerView and Skyline are two the most commonly used.",

keywords = "Data dependent acquisition, Data independent acquisition, MarkerView, Principal component analysis, SWATH-MS, Skyline, Statistical analysis",

author = "K. Frederick and P. Ciborowski",

year = "2016",

month = mar,

day = "22",

doi = "10.1016/B978-0-444-63688-1.00009-4",

language = "English (US)",

isbn = "9780444636881",

pages = "161--173",

booktitle = "Proteomic Profiling and Analytical Chemistry",

publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - SWATH-MS

T2 - Data Acquisition and Analysis

AU - Frederick, K.

AU - Ciborowski, P.

PY - 2016/3/22

Y1 - 2016/3/22

N2 - Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is an emerging proteomic platform for label free quantification. This platform is based on data independent acquisition (DIA) principle for simultaneous protein identification and quantification. SWATH-MS requires establishment of spectral library which is discussed in this chapter. SWATH-MS data analysis requires targeted data extraction, data normalization. Typical presentation of results from experiments using SWATH-MS is principal component analysis. Several pipelines of DIA analysis are available, however MarkerView and Skyline are two the most commonly used.

AB - Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) is an emerging proteomic platform for label free quantification. This platform is based on data independent acquisition (DIA) principle for simultaneous protein identification and quantification. SWATH-MS requires establishment of spectral library which is discussed in this chapter. SWATH-MS data analysis requires targeted data extraction, data normalization. Typical presentation of results from experiments using SWATH-MS is principal component analysis. Several pipelines of DIA analysis are available, however MarkerView and Skyline are two the most commonly used.

KW - Data dependent acquisition

KW - Data independent acquisition

KW - MarkerView

KW - Principal component analysis

KW - SWATH-MS

KW - Skyline

KW - Statistical analysis

UR - http://www.scopus.com/inward/record.url?scp=84969593578&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969593578&partnerID=8YFLogxK

U2 - 10.1016/B978-0-444-63688-1.00009-4

DO - 10.1016/B978-0-444-63688-1.00009-4

M3 - Chapter

AN - SCOPUS:84969593578

SN - 9780444636881

SP - 161

EP - 173

BT - Proteomic Profiling and Analytical Chemistry

PB - Elsevier Inc.

ER -

SWATH-MS: Data Acquisition and Analysis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this