Synchronization of drosophila cells in culture

Angie Rizzino, Alan B. Blumenthal

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalIn Vitro
Volume14
Issue number5
DOIs
StatePublished - May 1978

Keywords

  • Drosophila
  • S phase
  • cell cycle
  • cell lines
  • cell synchrony

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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