Synchronization of drosophila cells in culture

Angie Rizzino; Alan B. Blumenthal

doi:10.1007/BF02616105

Synchronization of drosophila cells in culture

Angie Rizzino, Alan B. Blumenthal

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We synchronized Drosophila cell lines (Schneider's line 2 and K_c) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G₂=6 to 8 hr; M=0.4 hr) were similar to those of K_c cells.

Original language	English (US)
Pages (from-to)	437-442
Number of pages	6
Journal	In Vitro
Volume	14
Issue number	5
DOIs	https://doi.org/10.1007/BF02616105
State	Published - May 1978

Keywords

Drosophila
S phase
cell cycle
cell lines
cell synchrony

ASJC Scopus subject areas

Biotechnology
Plant Science

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10.1007/BF02616105

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abstract = "We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.",

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AB - We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.

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KW - cell synchrony

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