Synchronization of drosophila cells in culture

Angie Rizzino; Alan B. Blumenthal

doi:10.1007/BF02616105

Synchronization of drosophila cells in culture

Angie Rizzino, Alan B. Blumenthal

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We synchronized Drosophila cell lines (Schneider's line 2 and K_c) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G₂=6 to 8 hr; M=0.4 hr) were similar to those of K_c cells.

Original language	English (US)
Pages (from-to)	437-442
Number of pages	6
Journal	In Vitro
Volume	14
Issue number	5
DOIs	https://doi.org/10.1007/BF02616105
State	Published - May 1978
Externally published	Yes

Keywords

Drosophila
S phase
cell cycle
cell lines
cell synchrony

ASJC Scopus subject areas

Biotechnology
Plant Science

Access to Document

10.1007/BF02616105

Cite this

@article{5065399463af41ba902f7cfa69be44b3,

title = "Synchronization of drosophila cells in culture",

abstract = "We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.",

keywords = "Drosophila, S phase, cell cycle, cell lines, cell synchrony",

author = "Angie Rizzino and Blumenthal, {Alan B.}",

year = "1978",

month = may,

doi = "10.1007/BF02616105",

language = "English (US)",

volume = "14",

pages = "437--442",

journal = "In Vitro",

issn = "0073-5655",

publisher = "Tissue Culture Association",

number = "5",

}

TY - JOUR

T1 - Synchronization of drosophila cells in culture

AU - Rizzino, Angie

AU - Blumenthal, Alan B.

PY - 1978/5

Y1 - 1978/5

N2 - We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.

AB - We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.

KW - Drosophila

KW - S phase

KW - cell cycle

KW - cell lines

KW - cell synchrony

UR - http://www.scopus.com/inward/record.url?scp=0017857573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017857573&partnerID=8YFLogxK

U2 - 10.1007/BF02616105

DO - 10.1007/BF02616105

M3 - Article

C2 - 352915

AN - SCOPUS:0017857573

SN - 0073-5655

VL - 14

SP - 437

EP - 442

JO - In Vitro

JF - In Vitro

IS - 5

ER -

Synchronization of drosophila cells in culture

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this