Abstract
We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S=14 to 16 hr; G2=6 to 8 hr; M=0.4 hr) were similar to those of Kc cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 437-442 |
| Number of pages | 6 |
| Journal | In Vitro |
| Volume | 14 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1978 |
Keywords
- Drosophila
- S phase
- cell cycle
- cell lines
- cell synchrony
ASJC Scopus subject areas
- Biotechnology
- Plant Science
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Cite this
Rizzino, A., & Blumenthal, A. B. (1978). Synchronization of drosophila cells in culture. In Vitro, 14(5), 437-442. https://doi.org/10.1007/BF02616105