Synthesis and fluorescent characteristics of imidazole-indocyanine green conjugates

Christopher Pavlik, Nrusingh C. Biswal, Faith Corbo Gaenzler, Martha D. Morton, Liisa T. Kuhn, Kevin P. Claffey, Quing Zhu, Michael B. Smith

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We have successfully synthesized imidazole-dye conjugates by linking imidazole and nitroimidazoleacetic acids to an indocyanine green (ICG) carboxylic acid derivative, using an ethanolamine linker. These dye-conjugates show absorbance peaks at 754-756 nm and fluorescence peaks at 780 nm. The dye-conjugates show a blue shift of 25 nm and 30 nm in the absorption and fluorescence spectra respectively when compared to that of standard cardiogreen. There is no change in absorption and fluorescence spectral profiles between the ICG derivative and imidazole conjugates. The extinction coefficients of new ICG derivative and imidazole conjugates are 1.8 times higher than that of standard ICG. The relative quantum yields of the new compounds are 4.5-5.5 times higher than that of the Sigma-Aldrich's ICG. The dyes are tested for hypoxia in-vitro with 4T1 luc cell lines and it is found that the cells treated with 2-nitroimidazole ICG show a contrast of fluorescence signal of 2.5-3.0 for the cells under hypoxic to that of cells under normoxic. However pure ICG shows no significant difference between hypoxic and normoxic cells.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalDyes and Pigments
Volume89
Issue number1
DOIs
StatePublished - Apr 2011

Keywords

  • Dye
  • Fluorescence
  • Hypoxia
  • Indocyanine green
  • Near infrared
  • Nitroimidazole

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

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    Pavlik, C., Biswal, N. C., Gaenzler, F. C., Morton, M. D., Kuhn, L. T., Claffey, K. P., Zhu, Q., & Smith, M. B. (2011). Synthesis and fluorescent characteristics of imidazole-indocyanine green conjugates. Dyes and Pigments, 89(1), 9-15. https://doi.org/10.1016/j.dyepig.2010.08.008