Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter

Jacob B. Griffin, J. Steven Stanley, Janos Zempleni

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

In mammalian cells, biotin is covalently attached to carboxylases and histones and is required for cell proliferation and function. Cellular uptake of biotin (as well as pantothenic acid and lipoic acid) is mediated by the sodium-dependent multivitamin transporter, SMVT. Studies of cellular biotin homeostasis have been hampered by the lack of an antibody to SMVT. Here, we describe the synthesis of a rabbit polyclonal antibody to human SMVT. Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT.

Original languageEnglish (US)
Pages (from-to)195-198
Number of pages4
JournalInternational Journal for Vitamin and Nutrition Research
Volume72
Issue number4
DOIs
StatePublished - Jan 1 2002

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Keywords

  • Biotin
  • Human
  • Lipoic acid
  • Pantothenic acid
  • Polyclonal antibody
  • Transport

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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