Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

Amarnath Natarajan; Nathan Moerke; Yun Hua Fan; Han Chen; William J. Christ; Gerhard Wagner; Jose A. Halperin

doi:10.1016/j.bmcl.2004.02.090

Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

Amarnath Natarajan, Nathan Moerke, Yun Hua Fan, Han Chen, William J. Christ, Gerhard Wagner, Jose A. Halperin

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Binding of eIF4E to the cap structure (m⁷GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.

Original language	English (US)
Pages (from-to)	2657-2660
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	14
Issue number	10
DOIs	https://doi.org/10.1016/j.bmcl.2004.02.090
State	Published - May 17 2004
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2004.02.090

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title = "Synthesis of fluorescein labeled 7-methylguanosinemonophosphate",

abstract = "Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.",

author = "Amarnath Natarajan and Nathan Moerke and Fan, {Yun Hua} and Han Chen and Christ, {William J.} and Gerhard Wagner and Halperin, {Jose A.}",

note = "Funding Information: The authors wish to thank Drs. Daniel C. Tosteson, and Magdalena T. Tosteson for continuous support, and Dr. Michael Chorev for comments and suggestions. This work was supported in part by NIH/NCDDG (5 U19 CA87427). ",

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T1 - Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

AU - Natarajan, Amarnath

AU - Moerke, Nathan

AU - Fan, Yun Hua

AU - Chen, Han

AU - Christ, William J.

AU - Wagner, Gerhard

AU - Halperin, Jose A.

N1 - Funding Information: The authors wish to thank Drs. Daniel C. Tosteson, and Magdalena T. Tosteson for continuous support, and Dr. Michael Chorev for comments and suggestions. This work was supported in part by NIH/NCDDG (5 U19 CA87427).

PY - 2004/5/17

Y1 - 2004/5/17

N2 - Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.

AB - Binding of eIF4E to the cap structure (m7GpppN) plays a critical role in mRNA translation. To study the interaction between eIF4E and cap, and to identify small molecule inhibitors of their binding, we synthesized a fluorescent-labeled cap analogue and used it to develop a fluorescence- polarization assay. This preliminary communication describes the synthesis of a fluorescein labeled 7-methylguanosinemonophosphate, and its dose dependent binding to purified human eIF4E as demonstrated by the fluorescence polarization assay.

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Synthesis of fluorescein labeled 7-methylguanosinemonophosphate

Abstract

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