Abstract
Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
Original language | English (US) |
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Pages (from-to) | 101-104 |
Number of pages | 4 |
Journal | Diagnostic Microbiology and Infectious Disease |
Volume | 59 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2007 |
Externally published | Yes |
Keywords
- Cervical cancer
- Genotyping
- Human papillomavirus
- Synthetic genes
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases