Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela; Catalina Amador; Manuel A. Patarroyo

doi:10.1016/j.diagmicrobio.2007.04.003

Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela, Catalina Amador, Manuel A. Patarroyo

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

Original language	English (US)
Pages (from-to)	101-104
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.04.003
State	Published - Sep 2007
Externally published	Yes

Keywords

Cervical cancer
Genotyping
Human papillomavirus
Synthetic genes

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2007.04.003

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Cite this

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abstract = "Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.",

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AU - Amador, Catalina

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