Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela; Catalina Amador; Manuel A. Patarroyo

doi:10.1016/j.diagmicrobio.2007.04.003

Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela, Catalina Amador, Manuel A. Patarroyo

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

Original language	English (US)
Pages (from-to)	101-104
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.04.003
State	Published - Sep 2007
Externally published	Yes

Keywords

Cervical cancer
Genotyping
Human papillomavirus
Synthetic genes

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/j.diagmicrobio.2007.04.003

Cite this

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title = "Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays",

abstract = "Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.",

keywords = "Cervical cancer, Genotyping, Human papillomavirus, Synthetic genes",

author = "Karen Orjuela and Catalina Amador and Patarroyo, {Manuel A.}",

note = "Funding Information: We would like to thank the President of Colombia's office, Tricia Hadley, for patiently reviewing the manuscript, Sandra Milena Camargo for technical support, and Professor Manuel Elkin Patarroyo for invaluable comments. This work was supported by Colciencias (contract #RC-0060-2006). ",

year = "2007",

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doi = "10.1016/j.diagmicrobio.2007.04.003",

language = "English (US)",

volume = "59",

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journal = "Diagnostic Microbiology and Infectious Disease",

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T2 - a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

AU - Orjuela, Karen

AU - Amador, Catalina

AU - Patarroyo, Manuel A.

N1 - Funding Information: We would like to thank the President of Colombia's office, Tricia Hadley, for patiently reviewing the manuscript, Sandra Milena Camargo for technical support, and Professor Manuel Elkin Patarroyo for invaluable comments. This work was supported by Colciencias (contract #RC-0060-2006).

PY - 2007/9

Y1 - 2007/9

N2 - Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

AB - Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.

KW - Cervical cancer

KW - Genotyping

KW - Human papillomavirus

KW - Synthetic genes

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Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Abstract

Keywords

ASJC Scopus subject areas

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