@article{e8b4fa83eb7f42fcaef3bfe8ceb54302,
title = "Tapasin decreases immune responsiveness to a model tumor antigen",
abstract = "The T-cell response against cancer is dependent on the cell surface presentation of tumor-associated or tumor-specific peptides by major histocompatibility complex (MHC) class I molecules. We found that tapasin, a chaperone protein that normally assists in the assembly of MHC class I molecules, is undetectable in an unstimulated pancreatic tumor cell line, Panc02, and only very weakly expressed after γ-interferon stimulation. Transfection of tapasin into the Panc02 cells did not quantitatively increase MHC class I surface expression or detectably affect MHC class I association with peptide and β2-microglubulin (β2m). However, we found that transfected tapasin downregulated immune reactivity against a model tumor antigen, MUC1. Although tapasin has been previously shown by others to increase immune recognition of particular antigens, our results suggest that tapasin has a negative impact on the presentation of an immunodominant epitope from a specific model tumor antigen.",
keywords = "Antigen presentation, MHC, Pancreatic cancer, Tapasin, Tumor antigen",
author = "Turnquist, {Heth R.} and Kohlgraf, {Karl G.} and McIlhaney, {Mary M.} and {Lee Mosley}, R. and Hollingsworth, {Michael A.} and Solheim, {Joyce C.}",
note = "Funding Information: We thank Drs. Ted Hansen, Peter Van Endert, Andreas G. Grandea III, and Luc Van Kaer for gifts of Ab and cell lines and Dr. Ping Wang for the gift of the tapasin cDNA. We also gratefully acknowledge the assistance of the personnel of the Eppley Animal Facility and of the University of Nebraska Medical Center Cell Analysis Facility, as well as the help of Dr. Andrew Gawron and Nicole Burns with the mouse survival studies, and the technical support of Rachael Turnquist and Dannielle Braund. This work was supported by NIH Grant GM57428, a University of Nebraska Seed Grant, and an LB595/Cattlemen{\textquoteright}s Ball Grant (to J.C.S.), and by the Nebraska Research Initiative Program in Molecular Therapeutics and the NIH Specialized Program in Research Excellence grant P50 CA72712 (to J.C.S. and M.A.H.). H.R.T. and K.G.K. received support from NIH Training Grant T32 CA09476 and UNMC Graduate Fellowships, and H.R.T. also received support from a UNMC Presidential Fellowship.",
year = "2004",
month = jul,
doi = "10.1023/B:JOCI.0000029118.51587.d9",
language = "English (US)",
volume = "24",
pages = "462--470",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer",
number = "4",
}