TY - JOUR
T1 - Target Cell Expression of Suppressor of Cytokine Signaling-1 Prevents Diabetes in the NOD Mouse
AU - Flodström-Tullberg, Malin
AU - Yadav, Deepak
AU - Hägerkvist, Robert
AU - Tsai, Devin
AU - Secrest, Patrick
AU - Stotland, Alexandr
AU - Sarvetnick, Nora
PY - 2003/11
Y1 - 2003/11
N2 - Although lymphocyte infiltration and islet destruction are hallmarks of diabetes, the mechanisms of β-cell destruction are not fully understood. One issue that remains unresolved is whether cytokines play a direct role in β-cell death. We investigated whether β-cell cytokine signaling contributes to autoimmune type 1 diabetes. We demonstrated that NOD mice harboring β-cells expressing the suppressor of cytokine signaling-1 (SOCS-1), an inhibitor of Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling, have a markedly reduced incidence of diabetes. Similar to their non-transgenic (Tg) litter-mates, SOCS-1-Tg mice develop insulitis and their splenocytes transfer disease to NODscid recipients. Disease protection correlates with suppression of cytokine-induced STAT-1 phosphorylation in SOCS-1-expressing β-cells and with a reduced sensitivity of these cells to destruction by diabetogenic cells in vivo. Interestingly, lymphocytes recruited to the pancreas of SOCS-1-Tg mice transferred diabetes to NODscid recipients with a reduced efficiency, suggesting that the pancreatic environment in SOCS-1-Tg mice does not support the maintenance of functionally differentiated T-cells. These results suggest that cytokines contribute to the development of type 1 diabetes by acting directly on the target β-cell. Importantly, given that the SOCS-1-expressing mouse maintain normal blood glucose levels throughout life, this study also showed that SOCS-1 expression by β-cells can represent a promising strategy to prevent type 1 diabetes.
AB - Although lymphocyte infiltration and islet destruction are hallmarks of diabetes, the mechanisms of β-cell destruction are not fully understood. One issue that remains unresolved is whether cytokines play a direct role in β-cell death. We investigated whether β-cell cytokine signaling contributes to autoimmune type 1 diabetes. We demonstrated that NOD mice harboring β-cells expressing the suppressor of cytokine signaling-1 (SOCS-1), an inhibitor of Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling, have a markedly reduced incidence of diabetes. Similar to their non-transgenic (Tg) litter-mates, SOCS-1-Tg mice develop insulitis and their splenocytes transfer disease to NODscid recipients. Disease protection correlates with suppression of cytokine-induced STAT-1 phosphorylation in SOCS-1-expressing β-cells and with a reduced sensitivity of these cells to destruction by diabetogenic cells in vivo. Interestingly, lymphocytes recruited to the pancreas of SOCS-1-Tg mice transferred diabetes to NODscid recipients with a reduced efficiency, suggesting that the pancreatic environment in SOCS-1-Tg mice does not support the maintenance of functionally differentiated T-cells. These results suggest that cytokines contribute to the development of type 1 diabetes by acting directly on the target β-cell. Importantly, given that the SOCS-1-expressing mouse maintain normal blood glucose levels throughout life, this study also showed that SOCS-1 expression by β-cells can represent a promising strategy to prevent type 1 diabetes.
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U2 - 10.2337/diabetes.52.11.2696
DO - 10.2337/diabetes.52.11.2696
M3 - Article
C2 - 14578288
AN - SCOPUS:0242268904
SN - 0012-1797
VL - 52
SP - 2696
EP - 2700
JO - Diabetes
JF - Diabetes
IS - 11
ER -