TY - JOUR
T1 - Target of rapamycin (TOR)-like 1 kinase is involved in the control of polyphosphate levels and acidocalcisome maintenance in Trypanosoma brucei
AU - De Jesus, Teresa Cristina Leandro
AU - Tonelli, Renata Rosito
AU - Nardelli, Sheila C.
AU - Da Silva Augusto, Leonardo
AU - Motta, Maria Cristina M.
AU - Girard-Dias, Wendell
AU - Miranda, Kildare
AU - Ulrich, Paul
AU - Jimenez, Veronica
AU - Barquilla, Antonio
AU - Navarro, Miguel
AU - Docampo, Roberto
AU - Schenkman, Sergio
PY - 2010/7/30
Y1 - 2010/7/30
N2 - Target of rapamycin (TOR) kinases are highly conserved protein kinases that integrate signals from nutrients and growth factors to coordinate cell growth and cell cycle progression. It has been previously described that two TOR kinases control cell growth in the protozoan parasite Trypanosoma brucei, the causative agent of African trypanosomiasis. Here we studied an unusual TOR-like protein named TbTOR-like 1 containing a PDZ domain and found exclusively in kinetoplastids. TbTOR-like 1 localizes to unique cytosolic granules. After hyperosmotic stress, the localization of the protein shifts to the cell periphery, different from other organelle markers. Ablation of TbTOR-like 1 causes a progressive inhibition of cell proliferation, producing parasites accumulating in the S/G2 phase of the cell cycle. TbTOR-like 1 knocked down cells have an increased area occupied by acidic vacuoles, known as acidocalcisomes, and are enriched in polyphosphate and pyrophosphate. These results suggest that TbTOR-like 1 might be involved in the control of acidocalcisome and polyphosphate metabolism in T. brucei.
AB - Target of rapamycin (TOR) kinases are highly conserved protein kinases that integrate signals from nutrients and growth factors to coordinate cell growth and cell cycle progression. It has been previously described that two TOR kinases control cell growth in the protozoan parasite Trypanosoma brucei, the causative agent of African trypanosomiasis. Here we studied an unusual TOR-like protein named TbTOR-like 1 containing a PDZ domain and found exclusively in kinetoplastids. TbTOR-like 1 localizes to unique cytosolic granules. After hyperosmotic stress, the localization of the protein shifts to the cell periphery, different from other organelle markers. Ablation of TbTOR-like 1 causes a progressive inhibition of cell proliferation, producing parasites accumulating in the S/G2 phase of the cell cycle. TbTOR-like 1 knocked down cells have an increased area occupied by acidic vacuoles, known as acidocalcisomes, and are enriched in polyphosphate and pyrophosphate. These results suggest that TbTOR-like 1 might be involved in the control of acidocalcisome and polyphosphate metabolism in T. brucei.
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U2 - 10.1074/jbc.M110.120212
DO - 10.1074/jbc.M110.120212
M3 - Article
C2 - 20495004
AN - SCOPUS:77954916983
SN - 0021-9258
VL - 285
SP - 24131
EP - 24140
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 31
ER -