Targeted antimicrobial activity of a specific IgG-SMAP28 conjugate against Porphyromonas gingivalis in a mixed culture

Michael R. Franzman, Kindra K. Burnell, Farideh H. Dehkordi-Vakil, Janet M. Guthmiller, Deborah V. Dawson, Kim A. Brogden

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Antimicrobial peptides coupled to a ligand, receptor or antibody for a specific pathogenic bacteria could be used to develop narrow-spectrum pharmaceuticals with 'targeted' antimicrobial activity void of adverse reactions often associated with the use of broad-spectrum antibiotics. To assess the feasibility of this approach, in this study sheep myeloid antimicrobial peptide (SMAP) 28 was linked to affinity- and protein G-purified rabbit immunoglobulin G (IgG) antibodies specific to the outer surface of Porphyromonas gingivalis strain 381. The selective activity of the P. gingivalis IgG-SMAP28 conjugate was then assessed by adding it to an artificially generated microbial community containing P. gingivalis, Aggregatibacter actinomycetemcomitans and Peptostreptococcus micros. The specificity of the P. gingivalis IgG-SMAP28 conjugate in this mixed culture was concentration-dependent. The conjugate at 50 μg protein/mL lacked specificity and killed P. gingivalis, A. actinomycetemcomitans and P. micros. The conjugate at 20 μg protein/mL was more specific and killed P. gingivalis. This is an initial step to develop a selective antimicrobial agent that can eliminate a specific periodontal pathogen, such as P. gingivalis, from patients with periodontal disease without harming the normal commensal flora.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Volume33
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

Keywords

  • Aggregatibacter actinomycetemcomitans
  • Cathelicidins
  • Peptostreptococcus micros
  • Porphyromonas gingivalis
  • SMAP28
  • Targeted antimicrobial activity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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