Targeted reduction of oxytocin expression provides insights into its physiological roles

W. Scott Young, Emily Shepard, A. Courtney DeVries, Andreas Zimmer, Mary E. LaMarca, Edward I. Ginns, Janet Amico, Randy J. Nelson, Lothar Hennighausen, Kay Uwe Wagner

Research output: Contribution to journalReview articlepeer-review

42 Scopus citations

Abstract

Oxytocin is a nonapeptide hormone that participates in the regulation of parturition and lactation. It has also been implicated in various behaviors, such as mating and maternal, and memory. To investigate whether or not oxytocin (OT) is essential for any of these functions, we eliminated, by homologous recombination, most of the first intron and the last two exons of the OT gene in mice. Those exons encode the neurophysin portion of the oxytocin preprohormone which is hypothesized to help in the packaging and transport of OT. The homozygous mutant mice have no detectable neurophysin or processed oxytocin in the paraventricular nucleus, supraoptic nucleus or posterior pituitary. Interestingly, homozygous mutant males and females are fertile and the homozygous mutant females are able to deliver their litters. However, the pups do not successfully suckle and die within 24 hours without milk in their stomachs. OT injection into the dams or rescue with the rat OT gene restores the milk ejection in response to suckling. OT is also needed for post-partum alveolar proliferation. These results indicate an absolute requirement for oxytocin for successful milk ejection, but not for mating, parturition and milk production, in mice. Furthermore, homozygous mutant mice show reduced aggression in some tests.

Original languageEnglish (US)
Pages (from-to)231-240
Number of pages10
JournalAdvances in experimental medicine and biology
Volume449
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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