Techniques used to test native DNA

James Wisecarver

Research output: Contribution to journalArticlepeer-review

Abstract

Several techniques commonly are used to test native high-molecular- weight DNA obtained from cells and tissues, including the Southern blot, dot and slot blot procedures, and in situ hybridization of tissue sections with oligonucleotide probes. These techniques work well when there is an adequate amount of fresh tissue or cells available to provide a source for the intact DNA. These techniques involve separating, or denaturing, the double-stranded DNA into individual strands, and then applying a marked nucleotide probe and allowing it to hybridize to a complementary DNA sequence that may be present. These techniques are used to determine whether a particular DNA sequence is present.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalLaboratory medicine
Volume28
Issue number2
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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