Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

Harender S. Bisht; Devika Soundara Manickam; Yezi You; David Oupicky

doi:10.1021/bm0509927

Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

Harender S. Bisht, Devika Soundara Manickam, Yezi You, David Oupicky

Research output: Contribution to journal › Article › peer-review

103 Scopus citations

Abstract

End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.

Original language	English (US)
Pages (from-to)	1169-1178
Number of pages	10
Journal	Biomacromolecules
Volume	7
Issue number	4
DOIs	https://doi.org/10.1021/bm0509927
State	Published - Apr 2006
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1021/bm0509927

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title = "Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)",

abstract = "End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.",

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T1 - Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

AU - Bisht, Harender S.

AU - Manickam, Devika Soundara

AU - You, Yezi

AU - Oupicky, David

PY - 2006/4

Y1 - 2006/4

N2 - End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.

AB - End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.

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Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

Abstract

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