Temperature-controlled properties of DNA complexes with poly(ethylenlmine)-graft-poly(N-isopropylacrylamide)

Harender S. Bisht, Devika Soundara Manickam, Yezi You, David Oupicky

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Abstract

End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional free radical polymerization and was used to prepare graft copolymers with poly(ethylenimine) (PEI). The copolymers exhibited lower critical solution temperature (LCST) behavior between 30 and 32 °C and formed complexes with plasmid DNA. The LCST of the copolymers in the DNA complexes increased slightly to ∼34-35 °C. Cytotoxicity of the copolymers was evaluated by measuring lactate dehydrogenase (LDH) release from cells. The copolymers exhibited temperature-dependent toxicity, with higher levels of LDH release observed at temperatures above the LCST. Cellular uptake and transfection activity of the DNA complexes with the PEI-g-PNIPA copolymers were lower than those of the control PEI/DNA complexes at temperature below the LCST but increased to the PEI/DNA levels at temperatures above the LCST.

Original languageEnglish (US)
Pages (from-to)1169-1178
Number of pages10
JournalBiomacromolecules
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2006

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ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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