Testing protocols: Care of biological samples in a rural setting

Mary Beth Flanders Stepans; Susan L Wilhelm; T. Kim Rodehorst; Derek Smith; Clarann Weinert

doi:10.1177/1054773808327551

Testing protocols: Care of biological samples in a rural setting

Mary Beth Flanders Stepans, Susan L Wilhelm, T. Kim Rodehorst, Derek Smith, Clarann Weinert

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The purpose of this study is to evaluate collection, transport, and storage protocols of urine and air allergen samples from infants in a rural setting. Infant urine and aeroallergen samples (n = 21) are collected in the home setting in rural areas where time and distance to the central laboratory is a consideration. Urine samples are analyzed using ELISA tests after being transported using three different techniques: (a) ambient temperature, (b) dry ice, and (c) packaged on dry ice and shipped commercially. Following initial ELISA testing for levels of cytokines, urine samples are frozen at -70 degrees Celsius for 6 months. Samples are then reanalyzed for levels of cytokines. Dust samples are analyzed for levels of aeroallergens, stored at 4 degrees for 6 months, and reanalyzed. The integrity of samples varies by biomarker, shipment temperature, and storage time, creating a high degree of variability in results.

Original language	English (US)
Pages (from-to)	6-22
Number of pages	17
Journal	Clinical Nursing Research
Volume	18
Issue number	1
DOIs	https://doi.org/10.1177/1054773808327551
State	Published - Feb 2009

Keywords

Biomarkers
Infant
Respiratory
Rural
Sample collection

ASJC Scopus subject areas

General Nursing

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10.1177/1054773808327551

Cite this

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title = "Testing protocols: Care of biological samples in a rural setting",

abstract = "The purpose of this study is to evaluate collection, transport, and storage protocols of urine and air allergen samples from infants in a rural setting. Infant urine and aeroallergen samples (n = 21) are collected in the home setting in rural areas where time and distance to the central laboratory is a consideration. Urine samples are analyzed using ELISA tests after being transported using three different techniques: (a) ambient temperature, (b) dry ice, and (c) packaged on dry ice and shipped commercially. Following initial ELISA testing for levels of cytokines, urine samples are frozen at -70 degrees Celsius for 6 months. Samples are then reanalyzed for levels of cytokines. Dust samples are analyzed for levels of aeroallergens, stored at 4 degrees for 6 months, and reanalyzed. The integrity of samples varies by biomarker, shipment temperature, and storage time, creating a high degree of variability in results.",

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AU - Smith, Derek

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KW - Respiratory

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Testing protocols: Care of biological samples in a rural setting

Abstract

Keywords

ASJC Scopus subject areas

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