Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus)

Nancy A. Schable, Brant C. Faircloth, William E. Palmer, John P. Carroll, L. Wes Burger, Leonard A. Brennan, Cris Hagen, Travis C. Glenn

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We describe polymerase chain reaction (PCR) primers and conditions to amplify eight dinucleotide, one trinucleotide and 14 tetranucleotide microsatellite DNA loci isolated from the northern bobwhite (Colinus virginianus). The PCR primers were tested on 16 individuals collected from a population located within the Red Hills region of south Georgia and north Florida. The 23 primer pairs developed in this study yielded an average of 6.5 alleles per locus (range 2-11), an average observed heterozygosity of 0.47 (range 0.06-0.94) and average polymorphic information content of 0.60 (range 0.06-0.85).

Original languageEnglish (US)
Pages (from-to)415-419
Number of pages5
JournalMolecular Ecology Notes
Volume4
Issue number3
DOIs
StatePublished - Sep 2004
Externally publishedYes

Keywords

  • Colinus virginianus
  • Galliformes
  • Microsatellites
  • PCR
  • Primer
  • Quail
  • SSR

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • General Biochemistry, Genetics and Molecular Biology

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