Abstract
We describe polymerase chain reaction (PCR) primers and conditions to amplify eight dinucleotide, one trinucleotide and 14 tetranucleotide microsatellite DNA loci isolated from the northern bobwhite (Colinus virginianus). The PCR primers were tested on 16 individuals collected from a population located within the Red Hills region of south Georgia and north Florida. The 23 primer pairs developed in this study yielded an average of 6.5 alleles per locus (range 2-11), an average observed heterozygosity of 0.47 (range 0.06-0.94) and average polymorphic information content of 0.60 (range 0.06-0.85).
Original language | English (US) |
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Pages (from-to) | 415-419 |
Number of pages | 5 |
Journal | Molecular Ecology Notes |
Volume | 4 |
Issue number | 3 |
DOIs | |
State | Published - Sep 2004 |
Externally published | Yes |
Keywords
- Colinus virginianus
- Galliformes
- Microsatellites
- PCR
- Primer
- Quail
- SSR
ASJC Scopus subject areas
- Biochemistry
- Ecology
- General Biochemistry, Genetics and Molecular Biology