Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

Brant C. Faircloth, Allison Reid, Teresa Valentine, Hyung Eo Soo, Theron M. Terhune, Travis C. Glenn, William E. Palmer, Campbell Joseph Nairn, John P. Carroll

Research output: Contribution to journalArticlepeer-review

Abstract

We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

Original languageEnglish (US)
Pages (from-to)387-389
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number2
DOIs
StatePublished - Jun 2005
Externally publishedYes

Keywords

  • Bobcat
  • Dinucleotide repeats
  • Lynx rufus
  • Microsatellites
  • Primers
  • SSRs
  • Tetranucleotide repeats
  • Trinucleotide repeats

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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