Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

Brant C. Faircloth; Allison Reid; Teresa Valentine; Hyung Eo Soo; Theron M. Terhune; Travis C. Glenn; William E. Palmer; Campbell Joseph Nairn; John P. Carroll

doi:10.1111/j.1471-8286.2005.00936.x

Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

Brant C. Faircloth, Allison Reid, Teresa Valentine, Hyung Eo Soo, Theron M. Terhune, Travis C. Glenn, William E. Palmer, Campbell Joseph Nairn, John P. Carroll

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

Original language	English (US)
Pages (from-to)	387-389
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00936.x
State	Published - Jun 2005
Externally published	Yes

Keywords

Bobcat
Dinucleotide repeats
Lynx rufus
Microsatellites
Primers
SSRs
Tetranucleotide repeats
Trinucleotide repeats

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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10.1111/j.1471-8286.2005.00936.x

Cite this

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title = "Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)",

abstract = "We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).",

keywords = "Bobcat, Dinucleotide repeats, Lynx rufus, Microsatellites, Primers, SSRs, Tetranucleotide repeats, Trinucleotide repeats",

author = "Faircloth, {Brant C.} and Allison Reid and Teresa Valentine and Soo, {Hyung Eo} and Terhune, {Theron M.} and Glenn, {Travis C.} and Palmer, {William E.} and Nairn, {Campbell Joseph} and Carroll, {John P.}",

year = "2005",

month = jun,

doi = "10.1111/j.1471-8286.2005.00936.x",

language = "English (US)",

volume = "5",

pages = "387--389",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "2",

}

TY - JOUR

T1 - Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

AU - Faircloth, Brant C.

AU - Reid, Allison

AU - Valentine, Teresa

AU - Soo, Hyung Eo

AU - Terhune, Theron M.

AU - Glenn, Travis C.

AU - Palmer, William E.

AU - Nairn, Campbell Joseph

AU - Carroll, John P.

PY - 2005/6

Y1 - 2005/6

N2 - We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

AB - We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).

KW - Bobcat

KW - Dinucleotide repeats

KW - Lynx rufus

KW - Microsatellites

KW - Primers

KW - SSRs

KW - Tetranucleotide repeats

KW - Trinucleotide repeats

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DO - 10.1111/j.1471-8286.2005.00936.x

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SN - 1471-8278

VL - 5

SP - 387

EP - 389

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)

Abstract

Keywords

ASJC Scopus subject areas

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