The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis

Tran Tuan Hiep; Vladimir N. Kulikov; Vladimir N. Noskov; Genadij I. Sizonenko; Yuri O. Chernoff; Yuri I. Pavlov

doi:10.1002/yea.320091112

The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis

Tran Tuan Hiep, Vladimir N. Kulikov, Vladimir N. Noskov, Genadij I. Sizonenko, Yuri O. Chernoff, Yuri I. Pavlov

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.

Original language	English (US)
Pages (from-to)	1251-1258
Number of pages	8
Journal	Yeast
Volume	9
Issue number	11
DOIs	https://doi.org/10.1002/yea.320091112
State	Published - Nov 1993
Externally published	Yes

Keywords

Methylotrophic yeasts
gene homology analysis
phosphoribosyl‐5‐aminoimidazole‐carboxylase

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Genetics

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10.1002/yea.320091112

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title = "The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis",

abstract = "The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.",

keywords = "Methylotrophic yeasts, gene homology analysis, phosphoribosyl‐5‐aminoimidazole‐carboxylase",

author = "Hiep, {Tran Tuan} and Kulikov, {Vladimir N.} and Noskov, {Vladimir N.} and Sizonenko, {Genadij I.} and Chernoff, {Yuri O.} and Pavlov, {Yuri I.}",

year = "1993",

month = nov,

doi = "10.1002/yea.320091112",

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T1 - The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica

T2 - Cloning, sequencing and homology analysis

AU - Hiep, Tran Tuan

AU - Kulikov, Vladimir N.

AU - Noskov, Vladimir N.

AU - Sizonenko, Genadij I.

AU - Chernoff, Yuri O.

AU - Pavlov, Yuri I.

PY - 1993/11

Y1 - 1993/11

N2 - The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.

AB - The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli, chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.

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The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica: Cloning, sequencing and homology analysis

Abstract

Keywords

ASJC Scopus subject areas

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