The N(3) flavin anion is shown to be the active species in the surface-enhanced Raman (SER) spectra of riboflavin, lumiflavin, FMN, and FAD adsorbed on colloidal silver. Participation of the anion is verified by the pH dependence of the spectra, the near identity of flavin SER spectra in water and deuterium oxide colloids, and the absence of SER for flavins with covalently bound N(3) substituents. The SER spectrum of lumichrome is briefly discussed.
ASJC Scopus subject areas
- Colloid and Surface Chemistry