The annealing helicase HARP protects stalled replication forks

Yuan Jingsong, Gargi Ghosal, Chen Junjie

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

Mutations in HepA-related protein (HARP) are the only identified causes of Schimke immunoosseous dysplasia (SIOD). HARP has a unique annealing helicase activity in vitro, but the in vivo functional significance remains unknown. Here, we demonstrated that HARP is recruited to stalled replication forks via its direct interaction with Replication protein A (RPA). Cells with HARP depletion displayed increased spontaneous DNA damage and G2/M arrest, suggesting that HARP normally acts to stabilize stalled replication forks. Our data place the annealing helicase activity of HARP at replication forks and propose that SIOD syndrome may be caused by the destabilization of replication forks during cell proliferation.

Original languageEnglish (US)
Pages (from-to)2394-2399
Number of pages6
JournalGenes and Development
Volume23
Issue number20
DOIs
StatePublished - Oct 15 2009

Keywords

  • Annealing helicase
  • HARP
  • Replication fork
  • SIOD
  • SMARCAL1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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