TY - JOUR
T1 - The binding of 7,12-dimethylbenz[a]anthracene to mammary gland macromolecules in the hamster
T2 - Effects of enovid feeding or transplacental exposure to diethylstilboestrol
AU - Hertzog, P. J.
AU - Lawson, T. A.
AU - Gingell, R.
N1 - Funding Information:
This work was supported by USPHS Contract No. CP33278. The authors wish to thank Dr. A. Ross, Eppley Institute, for helpful advice and discussion.
PY - 1981/10
Y1 - 1981/10
N2 - The covalent binding of 7,12-[3H]dimethylbenz[a]anthracene ([3H]DMBA) to mammary gland macromolecules was studied in hamsters fed a contraceptive mixture, Enovid, those exposed transplacentally to diethylstilboestrol (DES), and controls. Compared with rats, hamsters are relatively resistant to DMBA mammary carcinogenesis, but susceptibility is increased by either of the above treatments with Enovid or DES. The amount of DMBA bound to DNA and protein was 4-5 times greater than to RNA, but only DNA binding was persistent. Fifty-three percent of the DNA-bound DMBA was still present after 8 days. The amount of DMBA bound to hamster mammary DNA and its persistence was similar to that found in rats. Neither Enovid nor DES treatment altered the levels of binding to mammary macromolecules, nor their persistence. These results indicate that the species differences in the susceptibility to DMBA-induced mammary carcinogenesis in hamsters and rats, and modification of the former by hormones, is not due to differences in the activation of carcinogens. The role of hormones such as prolactin in the promotion phase of mammary gland carcinogenesis may explain these differences.
AB - The covalent binding of 7,12-[3H]dimethylbenz[a]anthracene ([3H]DMBA) to mammary gland macromolecules was studied in hamsters fed a contraceptive mixture, Enovid, those exposed transplacentally to diethylstilboestrol (DES), and controls. Compared with rats, hamsters are relatively resistant to DMBA mammary carcinogenesis, but susceptibility is increased by either of the above treatments with Enovid or DES. The amount of DMBA bound to DNA and protein was 4-5 times greater than to RNA, but only DNA binding was persistent. Fifty-three percent of the DNA-bound DMBA was still present after 8 days. The amount of DMBA bound to hamster mammary DNA and its persistence was similar to that found in rats. Neither Enovid nor DES treatment altered the levels of binding to mammary macromolecules, nor their persistence. These results indicate that the species differences in the susceptibility to DMBA-induced mammary carcinogenesis in hamsters and rats, and modification of the former by hormones, is not due to differences in the activation of carcinogens. The role of hormones such as prolactin in the promotion phase of mammary gland carcinogenesis may explain these differences.
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U2 - 10.1016/0009-2797(81)90177-0
DO - 10.1016/0009-2797(81)90177-0
M3 - Article
C2 - 6169461
AN - SCOPUS:0019481442
SN - 0009-2797
VL - 37
SP - 199
EP - 205
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 1-2
ER -