TY - JOUR
T1 - The biological activity of cationic liposomes in drug delivery and toxicity test in animal models
AU - Qi, Panpan
AU - Cao, Mei
AU - Song, Liju
AU - Chen, Chong
AU - Liu, Mingdong
AU - Li, Ningzhe
AU - Wu, Daoyan
AU - Peng, Jingshan
AU - Hu, Guoku
AU - Zhao, Jian
N1 - Funding Information:
Panpan Qi and Mei Cao contributed equally to this work. This work was supported by the National Natural Science Foundation of China (grant number 31270175 ). This was also supported by the Program for New Century Excellent Talents in University (grant number NCET-13-0397 ) and the Fundamental Research Funds for the Central Universities (grant number 2013SCU04B14 ).
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - In the study we made use of DOTAP (1,2-dioleoyl-3-trimethylammonium), DOPE (1,2-dioleoyl-snglycero-3-phosphoethanolamine) and PEG-PE (polyethylene glycol- polyethylene) to make cationic PEG-liposomes by ultrasonic dispersion method. The plasmid pGPU6 combined with cationic PEG-liposomes or Liopofectamin 2000 was used to transfect PC3 cells to judge the transfection efficiency. HE staining showed that the pGUP6-shAurora B plasmid/liposomes complex could significantly inhibit tumor growth in mice tumor model. The results indicated that there was no remarkable difference between the homemade liposomes and Lipofectamin 2000 after transfection, with transfection efficiency over 80%. And the homemade liposomes also had high transfection efficiency in vivo. No significant side effects were observed on weight, coat condition, behavior or appetite and the life span of mice treated with pGPU6-shAurora B were extended. Beyond that, there were no differences in mortality or in pathological changes to the heart, liver, spleen, lungs and kidneys among all the mice.
AB - In the study we made use of DOTAP (1,2-dioleoyl-3-trimethylammonium), DOPE (1,2-dioleoyl-snglycero-3-phosphoethanolamine) and PEG-PE (polyethylene glycol- polyethylene) to make cationic PEG-liposomes by ultrasonic dispersion method. The plasmid pGPU6 combined with cationic PEG-liposomes or Liopofectamin 2000 was used to transfect PC3 cells to judge the transfection efficiency. HE staining showed that the pGUP6-shAurora B plasmid/liposomes complex could significantly inhibit tumor growth in mice tumor model. The results indicated that there was no remarkable difference between the homemade liposomes and Lipofectamin 2000 after transfection, with transfection efficiency over 80%. And the homemade liposomes also had high transfection efficiency in vivo. No significant side effects were observed on weight, coat condition, behavior or appetite and the life span of mice treated with pGPU6-shAurora B were extended. Beyond that, there were no differences in mortality or in pathological changes to the heart, liver, spleen, lungs and kidneys among all the mice.
KW - Cationic liposomes
KW - DOPE
KW - DOTAP
KW - PEG-PE
KW - Toxicity assessment
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U2 - 10.1016/j.etap.2016.09.015
DO - 10.1016/j.etap.2016.09.015
M3 - Article
C2 - 27694054
AN - SCOPUS:84989179801
SN - 1382-6689
VL - 47
SP - 159
EP - 164
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
ER -