TY - JOUR
T1 - The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport
AU - Chowdhury, S. I.
AU - Brum, M. C S
AU - Coats, C.
AU - Doster, A.
AU - Wei, Huiyong
AU - Jones, C.
N1 - Funding Information:
This work was supported by USDA grants to S.I. Chowdhury ( 2009-35204-05200 ) and 2004-35204-14657 ) and C. Jones ( 08-00891 and 09-01653 ).
PY - 2011/9/28
Y1 - 2011/9/28
N2 - In this study, we examined the functional role of bovine herpesvirus type 1 (BHV-1) Us9 acidic domain residues 83-90 in the anterograde axonal transport of the virus in calves (natural host), rabbits, and in cultured neurons. A mutant virus strain lacking Us9 residues 83-90 (BHV-1 Us9 Δ83-90) and the rescued virus (BHV-1 Us9 R83-90) replicated efficiently in the nasal and ocular epithelium during primary infection and established latency in the trigeminal ganglia (TG). However, upon reactivation from latency, only the BHV-1 Us9 R83-90 virus was detected in nasal and ocular swabs of animals. In compartmentalized, rabbit primary dorsal root ganglia (DRG) neuron cultures, the Us9-deleted BHV-1, BHV-1 Us9 Δ83-90 and BHV-1 Us9 R83-90 viruses were transported efficiently in the retrograde direction. However, only the BHV-1 Us9 R83-90 virus was transported in an anterograde direction. These studies suggested that the Us9 acidic domain residues located between 83 and 90 were required for axonal anterograde transport.
AB - In this study, we examined the functional role of bovine herpesvirus type 1 (BHV-1) Us9 acidic domain residues 83-90 in the anterograde axonal transport of the virus in calves (natural host), rabbits, and in cultured neurons. A mutant virus strain lacking Us9 residues 83-90 (BHV-1 Us9 Δ83-90) and the rescued virus (BHV-1 Us9 R83-90) replicated efficiently in the nasal and ocular epithelium during primary infection and established latency in the trigeminal ganglia (TG). However, upon reactivation from latency, only the BHV-1 Us9 R83-90 virus was detected in nasal and ocular swabs of animals. In compartmentalized, rabbit primary dorsal root ganglia (DRG) neuron cultures, the Us9-deleted BHV-1, BHV-1 Us9 Δ83-90 and BHV-1 Us9 R83-90 viruses were transported efficiently in the retrograde direction. However, only the BHV-1 Us9 R83-90 virus was transported in an anterograde direction. These studies suggested that the Us9 acidic domain residues located between 83 and 90 were required for axonal anterograde transport.
KW - Anterograde neuronal transport
KW - BHV-1 envelope protein Us9
KW - Compartmentalized primary neuronal cultures
KW - Latency and reactivation
KW - Trigeminal ganglia
UR - http://www.scopus.com/inward/record.url?scp=80051819724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051819724&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2011.05.012
DO - 10.1016/j.vetmic.2011.05.012
M3 - Article
C2 - 21640524
AN - SCOPUS:80051819724
SN - 0378-1135
VL - 152
SP - 270
EP - 279
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -