The chlorophyll fluorescence ratio F735F700 as an accurate measure of the chlorophyll content in plants

Anatoly A. Gitelson, Claus Buschmann, Hartmut K. Lichtenthaler

Research output: Contribution to journalArticlepeer-review

190 Scopus citations


A remote sensing technique is presented to estimate the chlorophyll content in higher plants. The ratio between chlorophyll fluorescence at 735 nm and in the range 700-710 nm, F735/F700 was found to be linearly proportional to the chlorophyll content (with determination coefficient, r2, more than 0.95), and, thus, this ratio can be used as a precise indicator of chlorophyll content in plant leaves. This new chlorophyll fluorescence ratio indicates chlorophyll levels with high precision- the error in chlorophyll prediction over a wide range of chlorophyll content (from 41 to 675 mg m-2) was less than 40 mg m-2. The technique was tested and validated in three plant species: beech (Fagus sylvatica L.), elm (Ulmus minor Miller), and wild vine (Parthenocissus tricuspidata L.).

Original languageEnglish (US)
Pages (from-to)296-302
Number of pages7
JournalRemote Sensing of Environment
Issue number3
StatePublished - Sep 1999

ASJC Scopus subject areas

  • Soil Science
  • Geology
  • Computers in Earth Sciences

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