The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

P. J. Brixey; C. Guda; H. Daniell

doi:10.1023/A:1018371405675

The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

P. J. Brixey, C. Guda, H. Daniell

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

Original language	English (US)
Pages (from-to)	395-400
Number of pages	6
Journal	Biotechnology Letters
Volume	19
Issue number	4
DOIs	https://doi.org/10.1023/A:1018371405675
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein",

abstract = "The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.",

author = "Brixey, {P. J.} and C. Guda and H. Daniell",

note = "Funding Information: This research was supported in part by the NIH grant #GM16551–02 to PJB and HD, and two USDA grants #93–37311 and #95–02770 to HD. The authors are thankful to Dr. Dan Urry (University of Alabama at Birmingham) for providing coding sequences of the polymer genes used in this study.",

year = "1997",

doi = "10.1023/A:1018371405675",

language = "English (US)",

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journal = "Biotechnology Letters",

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T1 - The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

AU - Brixey, P. J.

AU - Guda, C.

AU - Daniell, H.

N1 - Funding Information: This research was supported in part by the NIH grant #GM16551–02 to PJB and HD, and two USDA grants #93–37311 and #95–02770 to HD. The authors are thankful to Dr. Dan Urry (University of Alabama at Birmingham) for providing coding sequences of the polymer genes used in this study.

PY - 1997

Y1 - 1997

N2 - The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

AB - The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

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The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

Abstract

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