The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-β-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology