The chronic infusion of nicotine into the developing chick embryo does not alter tensity of (-)-[3H]nicotine-binding sites or vestibular function

Richard L. Roll, Timothy A. Jones, Neal L. Benowitz, Barbara J. Morley

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

(-)-Nicotine (1.2 mg/day) or saline was infused into chick embryos (Gallus domesticus) for 10 days beginning 12 h beyond the eight day of incubation (E8 + 12 h). Twelve h beyond the eightenenth day if incubation (E8 + 12 h), the eggs were opened to access the embryos and subcutaneous skull electrodes placed. Short latency vestibular thresholds and input/output fure determined to assess neurophysiological consequences of chronic nicotine administration. Samples of serum extraembryonic (amniotic and albumen) fluid were analyzed by gas chromatography-mass spectrometry to determine the levels of nicotine and its major metabolite, cotinine. The brains were removed and divided into diencephalon and mesencephalon and the density of (-)-[3H]nicotine binding sites in each brain area was measured. Nicotine and cotinine were found in the serum and extraembryonic fluid, but nicotinic receptors were not up-regulated in the brains of animals infused with nicotine in comparison to controls. Vestibular response thresholds also did not differ between nicotine-treated and control animals.

Original languageEnglish (US)
Pages (from-to)260-265
Number of pages6
JournalBrain Research
Volume604
Issue number1-2
DOIs
StatePublished - Feb 26 1993

Keywords

  • Chick
  • Cotinine
  • Nicotine
  • Nicotinic cholinergic receptor
  • Response threshold
  • Vestibular efferent
  • Vestibular system

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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