The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus

Tatiana Rochat; Chantal Bohn; Claire Morvan; Thao Nguyen Le Lam; Fareha Razvi; Adrien Pain; Claire Toffano-Nioche; Prishila Ponien; Annick Jacq; Eric Jacquet; Paul D. Fey; Daniel Gautheret; Philippe Bouloc

doi:10.1093/nar/gky584

The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus

Tatiana Rochat, Chantal Bohn, Claire Morvan, Thao Nguyen Le Lam, Fareha Razvi, Adrien Pain, Claire Toffano-Nioche, Prishila Ponien, Annick Jacq, Eric Jacquet, Paul D. Fey, Daniel Gautheret, Philippe Bouloc

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with arginine catabolism. In particular, RsaE targets the arginase rocF mRNA via direct interactions involving G-rich motifs. Two duplicated C-rich motifs of RsaE can independently downregulate rocF expression. The faster growth rate of rsaE compared to its parental strain in media containing amino acids as sole carbon source points to an underlying role for RsaE in amino acid catabolism. Collectively, the data support a model in which RsaE acts as a global regulator of functions associated with metabolic adaptation.

Original language	English (US)
Pages (from-to)	8803-8816
Number of pages	14
Journal	Nucleic acids research
Volume	46
Issue number	17
DOIs	https://doi.org/10.1093/nar/gky584
State	Published - 2018

ASJC Scopus subject areas

Genetics

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10.1093/nar/gky584

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The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. / Rochat, Tatiana; Bohn, Chantal; Morvan, Claire; Le Lam, Thao Nguyen; Razvi, Fareha; Pain, Adrien; Toffano-Nioche, Claire; Ponien, Prishila; Jacq, Annick; Jacquet, Eric; Fey, Paul D.; Gautheret, Daniel; Bouloc, Philippe.

In: Nucleic acids research, Vol. 46, No. 17, 2018, p. 8803-8816.

Research output: Contribution to journal › Article › peer-review

Rochat, Tatiana ; Bohn, Chantal ; Morvan, Claire ; Le Lam, Thao Nguyen ; Razvi, Fareha ; Pain, Adrien ; Toffano-Nioche, Claire ; Ponien, Prishila ; Jacq, Annick ; Jacquet, Eric ; Fey, Paul D. ; Gautheret, Daniel ; Bouloc, Philippe. / The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus. In: Nucleic acids research. 2018 ; Vol. 46, No. 17. pp. 8803-8816.

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title = "The conserved regulatory RNA RsaE down-regulates the arginine degradation pathway in Staphylococcus aureus",

abstract = "RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with arginine catabolism. In particular, RsaE targets the arginase rocF mRNA via direct interactions involving G-rich motifs. Two duplicated C-rich motifs of RsaE can independently downregulate rocF expression. The faster growth rate of rsaE compared to its parental strain in media containing amino acids as sole carbon source points to an underlying role for RsaE in amino acid catabolism. Collectively, the data support a model in which RsaE acts as a global regulator of functions associated with metabolic adaptation.",

author = "Tatiana Rochat and Chantal Bohn and Claire Morvan and {Le Lam}, {Thao Nguyen} and Fareha Razvi and Adrien Pain and Claire Toffano-Nioche and Prishila Ponien and Annick Jacq and Eric Jacquet and Fey, {Paul D.} and Daniel Gautheret and Philippe Bouloc",

note = "Funding Information: Agence Nationale pour la Recherche [ANR-2012-BLAN-1602 (Duplex-Omics); ANR-15-CE12-0003 (sRNA-Fit)]; Fondation pour la Recherche M{\'e}dicale [DBF20160635724]; National Institutes of Health [NIH/NIAID P01AI083211]. Funding for open access charge: Agence Nationale pour la Recherche [ANR-15-CE12-0003]. Conflict of interest statement. None declared.",

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AU - Rochat, Tatiana

AU - Bohn, Chantal

AU - Morvan, Claire

AU - Le Lam, Thao Nguyen

AU - Razvi, Fareha

AU - Pain, Adrien

AU - Toffano-Nioche, Claire

AU - Ponien, Prishila

AU - Jacq, Annick

AU - Jacquet, Eric

AU - Fey, Paul D.

AU - Gautheret, Daniel

AU - Bouloc, Philippe

N1 - Funding Information: Agence Nationale pour la Recherche [ANR-2012-BLAN-1602 (Duplex-Omics); ANR-15-CE12-0003 (sRNA-Fit)]; Fondation pour la Recherche Médicale [DBF20160635724]; National Institutes of Health [NIH/NIAID P01AI083211]. Funding for open access charge: Agence Nationale pour la Recherche [ANR-15-CE12-0003]. Conflict of interest statement. None declared.

PY - 2018

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