The distorted α2-adrenoceptor genotype distribution in F2 populations of dahl salt sensitive and resistant rats cross

Lu Sun, Melanie Chun, Shari McArdle, William Kimberling, William Pettinger

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To examine the role of the α2-adrenoceptor gene in the development of genetic hypertensive rats, we tested Dahl salt sensitive (S) and resistant rats (R) for the presence of a restriction fragment length polymorphism (RFLP) in that gene. An RFLP was found between the S and R rats with a human kidney cDNA α2-adrenoceptor probe (α2-C4) and Msp I restriction endonuclease. The α2-C4 probe detected two alleles, S and R, of 3.0 and 2.8 kb in size. The two strains of rats were each homozygous for their corresponding allele. The inheritance of the alleles was investigated by crossbreeding S and R rats and subsequent brother/sister mating of F1 rats. Two hundred and fifteen F2 rats were produced by breeding 14 pairs of F1 rats. An atypical distribution of α2-adrenoceptor genotypes was observed. We found a reduced number of the SS genotype in both males and females of the F2 generation. To investigate the mechanism of this distorted α2-adnenoceptor genotype distribution in the F2 rats of S and R cross, we backrossed the F1 rats to their S and R parents. The litter size and gender distribution were counted for each breeding colony. Analysis by chi-square test showed that there was no sex difference among the backcrosses. Also, there was no significant decrease in litter size. This excludes the possibilities of fetal demise of S homozygotes and intrauterine selection. Therefore the deficiency of SS genotype may be due to gene recombination or may not be due to the α2-adrenoceptor gene itself, but to the effect of other genes closely linked to the α2-adrenoceptor.

Original languageEnglish (US)
Pages (from-to)PL45-PL50
JournalLife Sciences
Volume53
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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