The effect of Co(III)(NH3)4ATP on the kinetics of beef heart mitochondrial ATPase

L. Steinke, S. M. Schuster

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Bidentate cobalt(III)tetraamine adenosine triphosphate [Co(NH3)4ATP] was investigated as an inhibitor of the beef heart mitochondrial F1-ATPase. The compound was found to have a mixed noncompetitive mechanism with a Ki of 0.4 mm and an α of 1.4 during ATP hydrolysis. Co(NH3)4ATP also noncompetitively inhibited ATP hydrolysis in the presence of bicarbonate. ITP hydrolysis was similarly affected. Co(NH3)4ATP was also used in dual inhibitor studies with adenylylimidodiphosphate (AMP-PNP) and azide; it was found to be mutually exclusive with AMP-PNP and azide. The compound also protected the F1 from modification by 4-chloro-7-nitroben-zofurazan. These results are discussed in terms of the regulation of the ATP hydrolysis reaction.

Original languageEnglish (US)
Pages (from-to)629-635
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume238
Issue number2
DOIs
StatePublished - May 1 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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