Abstract
Bidentate cobalt(III)tetraamine adenosine triphosphate [Co(NH3)4ATP] was investigated as an inhibitor of the beef heart mitochondrial F1-ATPase. The compound was found to have a mixed noncompetitive mechanism with a Ki of 0.4 mm and an α of 1.4 during ATP hydrolysis. Co(NH3)4ATP also noncompetitively inhibited ATP hydrolysis in the presence of bicarbonate. ITP hydrolysis was similarly affected. Co(NH3)4ATP was also used in dual inhibitor studies with adenylylimidodiphosphate (AMP-PNP) and azide; it was found to be mutually exclusive with AMP-PNP and azide. The compound also protected the F1 from modification by 4-chloro-7-nitroben-zofurazan. These results are discussed in terms of the regulation of the ATP hydrolysis reaction.
Original language | English (US) |
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Pages (from-to) | 629-635 |
Number of pages | 7 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 238 |
Issue number | 2 |
DOIs | |
State | Published - May 1 1985 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology