The effect of experimental conditions on the assessment of T cell immunomodulation by biological response modifiers (thymosin fraction five)

James E. Talmadge; Karen L. Benedict; Keith A. Uithoven; Barbara F. Lenz

doi:10.1016/0162-3109(84)90004-3

The effect of experimental conditions on the assessment of T cell immunomodulation by biological response modifiers (thymosin fraction five)

James E. Talmadge, Karen L. Benedict, Keith A. Uithoven, Barbara F. Lenz

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The coculture of spleen cells with specific antigens in the presence of thymosin fraction five (F5) results in the stimulation of the lymphocyte response. To observe the stimulation, a suboptimal responder-to-stimulator ratio must be utilized. Suboptimal assay conditions also are needed in immunization challenge studies in which thymosin F5 acts as an effective immunoadjuvant. The data reported here suggest that to study the efficiency of a biological response modifier, suboptimal assay conditions are best for observation of immunomodulation. We also report that thymosin F5, in addition to exhibiting adjuvantlike activity for T cells, is stimulatory in assays of mixed lymphocyte response and in the in vitro stimulation of cytotoxic T effector cells, following a mixed lymphocyte-tumor culture.

Original language	English (US)
Pages (from-to)	17-26
Number of pages	10
Journal	Immunopharmacology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/0162-3109(84)90004-3
State	Published - Feb 1984
Externally published	Yes

Keywords

Biological response modifiers
Immunomodulation
Thymosin fraction five

ASJC Scopus subject areas

Pharmacology

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10.1016/0162-3109(84)90004-3

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title = "The effect of experimental conditions on the assessment of T cell immunomodulation by biological response modifiers (thymosin fraction five)",

abstract = "The coculture of spleen cells with specific antigens in the presence of thymosin fraction five (F5) results in the stimulation of the lymphocyte response. To observe the stimulation, a suboptimal responder-to-stimulator ratio must be utilized. Suboptimal assay conditions also are needed in immunization challenge studies in which thymosin F5 acts as an effective immunoadjuvant. The data reported here suggest that to study the efficiency of a biological response modifier, suboptimal assay conditions are best for observation of immunomodulation. We also report that thymosin F5, in addition to exhibiting adjuvantlike activity for T cells, is stimulatory in assays of mixed lymphocyte response and in the in vitro stimulation of cytotoxic T effector cells, following a mixed lymphocyte-tumor culture.",

keywords = "Biological response modifiers, Immunomodulation, Thymosin fraction five",

author = "Talmadge, {James E.} and Benedict, {Karen L.} and Uithoven, {Keith A.} and Lenz, {Barbara F.}",

note = "Funding Information: This project has been funded, at least in part, with funds from the Department of Health and Human Services, contract number NO 1-CO-23910 with Program Resources, Inc.",

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T1 - The effect of experimental conditions on the assessment of T cell immunomodulation by biological response modifiers (thymosin fraction five)

AU - Talmadge, James E.

AU - Benedict, Karen L.

AU - Uithoven, Keith A.

AU - Lenz, Barbara F.

N1 - Funding Information: This project has been funded, at least in part, with funds from the Department of Health and Human Services, contract number NO 1-CO-23910 with Program Resources, Inc.

PY - 1984/2

Y1 - 1984/2

N2 - The coculture of spleen cells with specific antigens in the presence of thymosin fraction five (F5) results in the stimulation of the lymphocyte response. To observe the stimulation, a suboptimal responder-to-stimulator ratio must be utilized. Suboptimal assay conditions also are needed in immunization challenge studies in which thymosin F5 acts as an effective immunoadjuvant. The data reported here suggest that to study the efficiency of a biological response modifier, suboptimal assay conditions are best for observation of immunomodulation. We also report that thymosin F5, in addition to exhibiting adjuvantlike activity for T cells, is stimulatory in assays of mixed lymphocyte response and in the in vitro stimulation of cytotoxic T effector cells, following a mixed lymphocyte-tumor culture.

AB - The coculture of spleen cells with specific antigens in the presence of thymosin fraction five (F5) results in the stimulation of the lymphocyte response. To observe the stimulation, a suboptimal responder-to-stimulator ratio must be utilized. Suboptimal assay conditions also are needed in immunization challenge studies in which thymosin F5 acts as an effective immunoadjuvant. The data reported here suggest that to study the efficiency of a biological response modifier, suboptimal assay conditions are best for observation of immunomodulation. We also report that thymosin F5, in addition to exhibiting adjuvantlike activity for T cells, is stimulatory in assays of mixed lymphocyte response and in the in vitro stimulation of cytotoxic T effector cells, following a mixed lymphocyte-tumor culture.

KW - Biological response modifiers

KW - Immunomodulation

KW - Thymosin fraction five

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The effect of experimental conditions on the assessment of T cell immunomodulation by biological response modifiers (thymosin fraction five)

Abstract

Keywords

ASJC Scopus subject areas

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