The effect of nucleotide substitution on DNA denaturation profiles

I. V. Razlutskii, L. S. Shlyakhtenko, Yu L. Lyubchenko

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28+ 0.03 C upon the deletion of one GC pair. The melting of DNS fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.

Original languageEnglish (US)
Pages (from-to)6665-6676
Number of pages12
JournalNucleic acids research
Volume15
Issue number16
DOIs
StatePublished - Aug 25 1987
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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