The effect of nucleotide substitution on DNA denaturation profiles

I. V. Razlutskii; L. S. Shlyakhtenko; Yu L. Lyubchenko

doi:10.1093/nar/15.16.6665

The effect of nucleotide substitution on DNA denaturation profiles

I. V. Razlutskii, L. S. Shlyakhtenko, Yu L. Lyubchenko

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1 Scopus citations

Abstract

The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28+ 0.03 C upon the deletion of one GC pair. The melting of DNS fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.

Original language	English (US)
Pages (from-to)	6665-6676
Number of pages	12
Journal	Nucleic acids research
Volume	15
Issue number	16
DOIs	https://doi.org/10.1093/nar/15.16.6665
State	Published - Aug 25 1987
Externally published	Yes

ASJC Scopus subject areas

Genetics

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AU - Shlyakhtenko, L. S.

AU - Lyubchenko, Yu L.

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Y1 - 1987/8/25

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AB - The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28+ 0.03 C upon the deletion of one GC pair. The melting of DNS fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.

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The effect of nucleotide substitution on DNA denaturation profiles

Abstract

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