The effects of anesthesia on the excretion of an isotonic saline load in the rat

Thomas F. Knight, Steven Sansom, Lois Hawk, Seymour J. Frankfurt, Edward J. Weinman

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The ability to excrete a volume of isotonic saline equal to 10% of body weight infused over 60 min, was examined in awake rats and in rats anesthetized with 1 of the 2 agents most commonly used in renal clearance studies, Inactin or Nembutal. Rats anesthetized with Inactin excreted significantly less of the infused sodium during the period of infusion and in the 120-min post-infusion periods as compared to Nembutal-anesthetized rats or awake rats. Following saline infusion, there was a significantly greater decrease in serum protein concentration (25.5±4.7%) in rats anesthetized with Inactin, compared to that observed in the awake or Nembutal-treated rats. In a separate group of saline-infused awake rats, induction of anesthesia with Inactin resulted in a significant increase in hematocrit and a decrease in serum protein concentration. These studies suggest that Inactin anesthesia decreases the ability of the kidney to excrete a saline load and that, in studies of sodium excretion in the rat, especially if volume expansion is to be part of the experimental protocol, Nembutal rather than Inactin may be the anesthetic of choice.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalPflügers Archiv European Journal of Physiology
Issue number2
StatePublished - Feb 1978
Externally publishedYes


  • Anesthesia
  • Sodium excretion

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)


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