The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines

Donald W. Miller; Michelle Fontain; Carol Kolar; Terence Lawson

doi:10.1016/0304-3835(96)04384-4

The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines

Donald W. Miller, Michelle Fontain, Carol Kolar, Terence Lawson

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [³H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [³H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.

Original language	English (US)
Pages (from-to)	301-306
Number of pages	6
Journal	Cancer Letters
Volume	107
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(96)04384-4
State	Published - Oct 22 1996
Externally published	Yes

Keywords

Drug resistance
MRP
P-gp
Pancreatic adenocarcinoma

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(96)04384-4

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title = "The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines",

abstract = "The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [3H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [3H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.",

keywords = "Drug resistance, MRP, P-gp, Pancreatic adenocarcinoma",

author = "Miller, {Donald W.} and Michelle Fontain and Carol Kolar and Terence Lawson",

note = "Funding Information: This research was funded in part by grants from the American Cancer Society IRG-#165-F; SIG 16, NC1 Cancer Laboratory Core Grant CA 36727 and the Nebraska Cancer and Smoking Disease Research Program.",

year = "1996",

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doi = "10.1016/0304-3835(96)04384-4",

language = "English (US)",

volume = "107",

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T1 - The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines

AU - Miller, Donald W.

AU - Fontain, Michelle

AU - Kolar, Carol

AU - Lawson, Terence

N1 - Funding Information: This research was funded in part by grants from the American Cancer Society IRG-#165-F; SIG 16, NC1 Cancer Laboratory Core Grant CA 36727 and the Nebraska Cancer and Smoking Disease Research Program.

PY - 1996/10/22

Y1 - 1996/10/22

N2 - The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [3H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [3H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.

AB - The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [3H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [3H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.

KW - Drug resistance

KW - MRP

KW - P-gp

KW - Pancreatic adenocarcinoma

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JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

The expression of multidrug resistance-associated protein (MRP) in pancreatic adenocarcinoma cell lines

Abstract

Keywords

ASJC Scopus subject areas

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