Abstract
The presence of P-glycoprotein (P-gp) and multiple drug resistance-associated protein (MRP) was examined in four human pancreatic adenocarcinoma cell lines (PANC-1, BxPC-3, AsPC-1, and Capan-1). Cellular accumulation of rhodamine 123 and [3H]vincristine were used to determine functional activity of P-gp and MRP, respectively. None of the cells showed any evidence of P-gp in the rhodamine 123 cellular accumulation assays. In contrast, PANC-1, BxPC-3 and AsPC-1 did display an increased accumulation of [3H]vincristine following treatment with either cyclosporin A or verapamil. Western blot analysis confirmed the expression of MRP, and little, if any, measurable P-gp in the cell lysates. These studies suggest that intrinsic drug resistance in pancreatic duct cancer may be due in part to the presence of MRP.
Original language | English (US) |
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Pages (from-to) | 301-306 |
Number of pages | 6 |
Journal | Cancer Letters |
Volume | 107 |
Issue number | 2 |
DOIs | |
State | Published - Oct 22 1996 |
Externally published | Yes |
Keywords
- Drug resistance
- MRP
- P-gp
- Pancreatic adenocarcinoma
ASJC Scopus subject areas
- Oncology
- Cancer Research