TY - JOUR
T1 - The expression of prostatic acid phosphatase is transcriptionally regulated in human prostate carcinoma cells
AU - Garcia-Arenas, Renee
AU - Lin, Fen Fen
AU - Lin, Dinlii
AU - Jin, Li Ping
AU - C.-Y. Shih, Charles
AU - Chang, Chawnshang
AU - Lin, Ming Fong
N1 - Funding Information:
The authors thank the Word Processing Pool of the USC/Norris Cancer Center for secretarial assistance in manuscript preparation, and Dr. Guo-Jang Wu for his technical help. This investigation was supported in part by a grant from the National Cancer Institute CA521 12 to M.F.L. and CA55639 to C. Chang.
PY - 1995/4/28
Y1 - 1995/4/28
N2 - The expression of prostatic acid phosphatase (PAcP) in three human prostate carcinoma cell lines including LNCaP, DU 145 and PC-3, was studied to explore its potential role as a marker in the progression of prostate cancer. Although Southern blot analysis suggested the presence of PAcP gene in all three prostate carcinoma cell lines, the Northern blot analysis and the reverse transcriptase-polymerase chain reaction (RT-PCR) assay showed that PAcP mRNA can be detected only in LNCaP cells. As one of the major differences between LNCaP cells and PC-3 as well as DU 145 cells is the androgen-sensitivity of LNCaP cells, we then focused on the influence of PAcP expression by the presence of androgen receptor (AR) in hman AR cDNA-transfected PC-3 cells and high passages of LNCaP cells. The results demonstrated that the transfection of human AR cDNA into PC-3 cells did not have any detectable effect on the expression of PAcP. Further, in LNCaP cells, while the level of PAcP mRNA diminished upon passage, the AR mRNA level remained approximately the same. Together, these data suggested that the differential expression of PAcP in different prostate carcinoma cells including high passages of LNCaP cells may occur at the transcriptional level and may have little linkage to the expression of AR.
AB - The expression of prostatic acid phosphatase (PAcP) in three human prostate carcinoma cell lines including LNCaP, DU 145 and PC-3, was studied to explore its potential role as a marker in the progression of prostate cancer. Although Southern blot analysis suggested the presence of PAcP gene in all three prostate carcinoma cell lines, the Northern blot analysis and the reverse transcriptase-polymerase chain reaction (RT-PCR) assay showed that PAcP mRNA can be detected only in LNCaP cells. As one of the major differences between LNCaP cells and PC-3 as well as DU 145 cells is the androgen-sensitivity of LNCaP cells, we then focused on the influence of PAcP expression by the presence of androgen receptor (AR) in hman AR cDNA-transfected PC-3 cells and high passages of LNCaP cells. The results demonstrated that the transfection of human AR cDNA into PC-3 cells did not have any detectable effect on the expression of PAcP. Further, in LNCaP cells, while the level of PAcP mRNA diminished upon passage, the AR mRNA level remained approximately the same. Together, these data suggested that the differential expression of PAcP in different prostate carcinoma cells including high passages of LNCaP cells may occur at the transcriptional level and may have little linkage to the expression of AR.
KW - Androgen
KW - Differentiation antigen
KW - Prostate epithelium
KW - Prostatic acid phosphatase (human)
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U2 - 10.1016/0303-7207(95)03544-H
DO - 10.1016/0303-7207(95)03544-H
M3 - Article
C2 - 7649350
AN - SCOPUS:0029003236
SN - 0303-7207
VL - 111
SP - 29
EP - 37
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1
ER -