TY - JOUR
T1 - The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis
AU - Yu, Bin
AU - Bi, Liu
AU - Zheng, Binglian
AU - Ji, Lijuan
AU - Chevalier, David
AU - Agarwal, Manu
AU - Ramachandran, Vanitharani
AU - Li, Wanxiang
AU - Lagrange, Thierry
AU - Walker, John C.
AU - Chen, Xuemei
PY - 2008/7/22
Y1 - 2008/7/22
N2 - Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs. Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs. Promoter activity of MIR genes, however, is not affected by ddl mutations. DDL is an RNA binding protein and is able to interact with DCL1. In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis. We propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs.
AB - Proteins containing the forkhead-associated domain (FHA) are known to act in biological processes such as DNA damage repair, protein degradation, and signal transduction. Here we report that DAWDLE (DDL), an FHA domain-containing protein in Arabidopsis, acts in the biogenesis of miRNAs and endogenous siRNAs. Unlike mutants of genes known to participate in the processing of miRNA precursors, such as dcl1, hyponastic leaves1, and serrate, ddl mutants show reduced levels of pri-miRNAs as well as mature miRNAs. Promoter activity of MIR genes, however, is not affected by ddl mutations. DDL is an RNA binding protein and is able to interact with DCL1. In addition, we found that SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha. Therefore, we uncovered an evolutionarily conserved factor in miRNA biogenesis. We propose that DDL participates in miRNA biogenesis by facilitating DCL1 to access or recognize pri-miRNAs.
KW - DCL1
KW - Drosha
KW - SMAD
KW - microRNA
KW - siRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=48249158259&partnerID=8YFLogxK
U2 - 10.1073/pnas.0804218105
DO - 10.1073/pnas.0804218105
M3 - Article
C2 - 18632581
AN - SCOPUS:48249158259
SN - 0027-8424
VL - 105
SP - 10073
EP - 10078
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 29
ER -