The fluorescent antibody and indirect fluorescent antibody assays for diagnosing stunting syndrome of turkeys

D. L. Reynolds, J. Oesper, A. Ali

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The fluorescent antibody (FA) assay was developed for detecting the stunting syndrome agent (SSA) from intestinal tissue. Similarly, the indirect fluorescent antibody (IFA) assay was developed for detecting serum antibodies to SSA. Convalescent antiserum from turkeys orally immunized with SSA was found to be the primary antibody of choice for the FA assay. Intestinal jejunal samples from poults inoculated 3-4 days postinoculation (DPI) was found to be the best antigen source for the IFA assay. SSA was detected from the intestinal tracts of experimentally inoculated birds at 2 DPI with highest level of reactivity at 3 DPI by the FA assay. After 4 DPI the level of SSA infectivity of the intestines waned to low levels. Serum antibody was detected from experimentally inoculated birds as early as 7 DPI with all birds tested seroconverting by 12 DPI. These assays should prove useful for future studies concerning stunting syndrome.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalAvian diseases
Volume44
Issue number2
DOIs
StatePublished - 2000

Keywords

  • Fluorescent antibody
  • Indirect fluorescent antibody
  • Poult enteritis
  • Stunting syndrome
  • Stunting syndrome agent

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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