The gene-gun approach for transfection and labeling of cells in brain slices

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Biolistic transfection and diolistic labeling are techniques in which subcellular-sized particles, coated with DNA and lipophilic dyes, respectively, are propelled into cells. The gene-gun approach is particularly applicable for use on ex vivo organized tissue such as brain slices, where cells are not accessible for transfection with methods used in dissociated cell preparations. This simple and rapid method results in targeting of individual cells in a Golgi-like manner, allowing investigating structural and functional aspects of neuronal development.

Original languageEnglish (US)
Title of host publicationNeural Development
Subtitle of host publicationMethods and Protocols
Pages111-118
Number of pages8
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1018
ISSN (Print)1064-3745

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Keywords

  • Biolistics
  • Brain slices
  • Diolistics
  • Gene-gun
  • Lipophilic dyes
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Dunaevsky, A. (2013). The gene-gun approach for transfection and labeling of cells in brain slices. In Neural Development: Methods and Protocols (pp. 111-118). (Methods in Molecular Biology; Vol. 1018). https://doi.org/10.1007/978-1-62703-444-9-11