The genetic activity of N6-hydroxyadenine and 2-amino-N6-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae

Y. I. Pavlov, V. N. Noskov, E. K. Lange, E. V. Moiseeva, M. R. Pshenichnov, N. N. Khromov-Borisov

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The genetic activity of 2-amino-N6-hydroxyadenine or 2-amino-N-hydroxylaminopurine (AHA) and N6-hydroxyadenine or 6-N-hydroxylaminopurine (HAP) was studied in S. typhimurium, E. coli and Saccharomyces cerevisiae strains. AHA was a more potent mutagen for bacteria and a less potent mutagen for yeast than HAP. The mutagenic activity of analogs was not influenced by excision, mutagenic or double-strand DNA repair mutations. On the other hand, the uvrBdel mutation has a drastic effect on the mutagenicity and toxicity of both analogs in the Salmonella strains studied. HAP was a very potent mutagen in yeast with a low capability of inducing mitotic recombination contrary to common mutagens, possessed unique intergenic specificity and was able to induce mutations in diploids at rather high frequency.

Original languageEnglish (US)
Pages (from-to)33-46
Number of pages14
JournalMutation Research/Environmental Mutagenesis and Related Subjects
Volume253
Issue number1
DOIs
StatePublished - Aug 1991

Keywords

  • 2-Amino-N-hydroxylaminopurine
  • Base-pair substitution mutations
  • DNA repair
  • Escherichia coli
  • Gene conversion
  • Mutagenesis in diploid cells
  • N-Hydroxylaminopurine
  • Saccharomyces cerevisiae
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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