The genetic activity of N6-hydroxyadenine and 2-amino-N6-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae

Y. I. Pavlov; V. N. Noskov; E. K. Lange; E. V. Moiseeva; M. R. Pshenichnov; N. N. Khromov-Borisov

doi:10.1016/0165-1161(91)90343-7

The genetic activity of N⁶-hydroxyadenine and 2-amino-N⁶-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae

Y. I. Pavlov, V. N. Noskov, E. K. Lange, E. V. Moiseeva, M. R. Pshenichnov, N. N. Khromov-Borisov

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

The genetic activity of 2-amino-N⁶-hydroxyadenine or 2-amino-N-hydroxylaminopurine (AHA) and N⁶-hydroxyadenine or 6-N-hydroxylaminopurine (HAP) was studied in S. typhimurium, E. coli and Saccharomyces cerevisiae strains. AHA was a more potent mutagen for bacteria and a less potent mutagen for yeast than HAP. The mutagenic activity of analogs was not influenced by excision, mutagenic or double-strand DNA repair mutations. On the other hand, the uvrB^del mutation has a drastic effect on the mutagenicity and toxicity of both analogs in the Salmonella strains studied. HAP was a very potent mutagen in yeast with a low capability of inducing mitotic recombination contrary to common mutagens, possessed unique intergenic specificity and was able to induce mutations in diploids at rather high frequency.

Original language	English (US)
Pages (from-to)	33-46
Number of pages	14
Journal	Mutation Research/Environmental Mutagenesis and Related Subjects
Volume	253
Issue number	1
DOIs	https://doi.org/10.1016/0165-1161(91)90343-7
State	Published - Aug 1991
Externally published	Yes

Keywords

2-Amino-N-hydroxylaminopurine
Base-pair substitution mutations
DNA repair
Escherichia coli
Gene conversion
Mutagenesis in diploid cells
N-Hydroxylaminopurine
Saccharomyces cerevisiae
Salmonella typhimurium

ASJC Scopus subject areas

Toxicology
Genetics

Access to Document

10.1016/0165-1161(91)90343-7

Cite this

Pavlov, Y. I., Noskov, V. N., Lange, E. K., Moiseeva, E. V., Pshenichnov, M. R., & Khromov-Borisov, N. N. (1991). The genetic activity of N⁶-hydroxyadenine and 2-amino-N⁶-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae. Mutation Research/Environmental Mutagenesis and Related Subjects, 253(1), 33-46. https://doi.org/10.1016/0165-1161(91)90343-7

The genetic activity of N⁶-hydroxyadenine and 2-amino-N⁶-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae. / Pavlov, Y. I.; Noskov, V. N.; Lange, E. K. et al.
In: Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 253, No. 1, 08.1991, p. 33-46.

Research output: Contribution to journal › Article › peer-review

Pavlov, YI, Noskov, VN, Lange, EK, Moiseeva, EV, Pshenichnov, MR & Khromov-Borisov, NN 1991, 'The genetic activity of N⁶-hydroxyadenine and 2-amino-N⁶-hydroxyadenine in Escherichia coli, Salmonella typhimurium and Saccharomyces cerevisiae', Mutation Research/Environmental Mutagenesis and Related Subjects, vol. 253, no. 1, pp. 33-46. https://doi.org/10.1016/0165-1161(91)90343-7

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abstract = "The genetic activity of 2-amino-N6-hydroxyadenine or 2-amino-N-hydroxylaminopurine (AHA) and N6-hydroxyadenine or 6-N-hydroxylaminopurine (HAP) was studied in S. typhimurium, E. coli and Saccharomyces cerevisiae strains. AHA was a more potent mutagen for bacteria and a less potent mutagen for yeast than HAP. The mutagenic activity of analogs was not influenced by excision, mutagenic or double-strand DNA repair mutations. On the other hand, the uvrBdel mutation has a drastic effect on the mutagenicity and toxicity of both analogs in the Salmonella strains studied. HAP was a very potent mutagen in yeast with a low capability of inducing mitotic recombination contrary to common mutagens, possessed unique intergenic specificity and was able to induce mutations in diploids at rather high frequency.",

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