TY - JOUR
T1 - The genome of polymorphonuclear neutrophils maintains normal coding sequences
AU - Xiao, Fengxia
AU - Kim, Yeong C.
AU - Wen, Hongxiu
AU - Luo, Jiangtao
AU - Chen, Peixian
AU - Cowan, Kenneth
AU - Wang, San Ming
PY - 2013/11/8
Y1 - 2013/11/8
N2 - Genetic studies often use genomic DNA from whole blood cells, of which the majority are the polymorphonuclear myeloid cells. Those cells undergo dramatic change of nuclear morphology following cellular differentiation. It remains elusive if the nuclear morphological change accompanies sequence alternations from the intact genome. If such event exists, it will cause a serious problem in using such type of genomic DNA for genetic study as the sequences will not represent the intact genome in the host individuals. Using exome sequencing, we compared the coding regions between neutrophil, which is the major type of polymorphonuclear cells, and CD4+ T cell, which has an intact genome, from the same individual. The results show that exon sequences between the two cell types are essentially the same. The minor differences represented by the missed exons and base changes between the two cell types were validated to be mainly caused by experimental errors. Our study concludes that genomic DNA from whole blood cells can be safely used for genetic studies.
AB - Genetic studies often use genomic DNA from whole blood cells, of which the majority are the polymorphonuclear myeloid cells. Those cells undergo dramatic change of nuclear morphology following cellular differentiation. It remains elusive if the nuclear morphological change accompanies sequence alternations from the intact genome. If such event exists, it will cause a serious problem in using such type of genomic DNA for genetic study as the sequences will not represent the intact genome in the host individuals. Using exome sequencing, we compared the coding regions between neutrophil, which is the major type of polymorphonuclear cells, and CD4+ T cell, which has an intact genome, from the same individual. The results show that exon sequences between the two cell types are essentially the same. The minor differences represented by the missed exons and base changes between the two cell types were validated to be mainly caused by experimental errors. Our study concludes that genomic DNA from whole blood cells can be safely used for genetic studies.
UR - http://www.scopus.com/inward/record.url?scp=84892563156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84892563156&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0078685
DO - 10.1371/journal.pone.0078685
M3 - Article
C2 - 24250807
AN - SCOPUS:84892563156
SN - 1932-6203
VL - 8
JO - PloS one
JF - PloS one
IS - 11
M1 - e78685
ER -