@article{55ef085f64694124b5acfeec78ce9944,
title = "The Growth of Cells in Serum-Free Hormone-Supplemented Media",
abstract = "This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.",
author = "Jane Bottenstein and Izumi Hayashi and Sharon Hutchings and Hideo Masui and Jennie Mather and McClure, {Don B.} and Sugayuki Ohasa and Angie Rizzino and Gordon Sato and Ginette Serrero and Richard Wolfe and Reen Wu",
note = "Funding Information: Bovine crystalline insulin, human transferrin, and steroid hormones are purchased from either Sigma or Calbiochem. Epidermal growth factor (EGF) and fibroblast growth factor (FGF) are purchased from Collaborative Research, Waltham, Massachusetts. A gift of a somatomedin preparation from Dr. Knut Uthne, Kabi Co., Stockholm, Sweden made it possible to start this work. Gifts of EGF from Dr. Stanley Cohen, Vanderbilt University, and FGF from Dr. Denis Gospodarowicz, University of California Medical School, San Francisco, were helpful in the early stages of this work. Highly purified pituitary hormones and somatomedins were generously provided by Dr. Harold Papkoff of the University of California, San Francisco, and Dr. Judson Van Wyk, University of North Carolina, Chapel Hill, respectively. Partially purified pituitary hormones are obtained from the National Institutes of Health Hormone Distribution Program . We are very grateful to Dr. Brian Kimes of the NCI. Without his timely assistance, this work would not have been possible. This work was supported by grants from the National Science Foundation, NIH GM 17019, NIH CA 19731, NIH GM 17702, and NCI CA 18885.",
year = "1979",
month = jan,
day = "1",
doi = "10.1016/S0076-6879(79)58127-0",
language = "English (US)",
volume = "58",
pages = "94--109",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",
}