The Growth of Cells in Serum-Free Hormone-Supplemented Media

Jane Bottenstein; Izumi Hayashi; Sharon Hutchings; Hideo Masui; Jennie Mather; Don B. McClure; Sugayuki Ohasa; Angie Rizzino; Gordon Sato; Ginette Serrero; Richard Wolfe; Reen Wu

doi:10.1016/S0076-6879(79)58127-0

The Growth of Cells in Serum-Free Hormone-Supplemented Media

Jane Bottenstein, Izumi Hayashi, Sharon Hutchings, Hideo Masui, Jennie Mather, Don B. McClure, Sugayuki Ohasa, Angie Rizzino, Gordon Sato, Ginette Serrero, Richard Wolfe, Reen Wu

Research output: Contribution to journal › Article › peer-review

264 Scopus citations

Abstract

This chapter presents the practical details for the growth of cells in serum free hormone supplemented media. In the absence of serum, greater than usual care must be taken in preparation of the synthetic portion of the medium. Careful preparation of water is essential for consistent results with serum-free medium. Serum and even dialyzed serum can mask nutritional requirements of cells in culture. Commercially available powdered media are adequate for serum-free work although each batch must be checked for suitability. Serum also serves the function of a trypsin inhibitor in conventional tissue culture procedures. The serum may be necessary for the repair of trypsinization damage after subculture; or the residual serum left after its removal, even after one or more washes, may be furnishing unknown growth factors.

Original language	English (US)
Pages (from-to)	94-109
Number of pages	16
Journal	Methods in enzymology
Volume	58
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(79)58127-0
State	Published - Jan 1 1979
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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The Growth of Cells in Serum-Free Hormone-Supplemented Media. / Bottenstein, Jane; Hayashi, Izumi; Hutchings, Sharon; Masui, Hideo; Mather, Jennie; McClure, Don B.; Ohasa, Sugayuki; Rizzino, Angie; Sato, Gordon; Serrero, Ginette; Wolfe, Richard; Wu, Reen.

In: Methods in enzymology, Vol. 58, No. C, 01.01.1979, p. 94-109.

Research output: Contribution to journal › Article › peer-review

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