TY - JOUR
T1 - The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector
AU - Shan, Libo
AU - Oh, Hye Sook
AU - Chen, Jianfu
AU - Guo, Ming
AU - Zhou, Jianmin
AU - Alfano, James R.
AU - Collmer, Alan
AU - Jia, Xu
AU - Tang, Xiaoyan
PY - 2004/5
Y1 - 2004/5
N2 - Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF Pto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopF Pto encode a type III chaperone and a cognate effector, respectively. ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell. Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of the HopFPto protein to a low level in bacterial cells.
AB - Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF Pto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopF Pto encode a type III chaperone and a cognate effector, respectively. ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell. Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of the HopFPto protein to a low level in bacterial cells.
UR - http://www.scopus.com/inward/record.url?scp=1942504720&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1942504720&partnerID=8YFLogxK
U2 - 10.1094/MPMI.2004.17.5.447
DO - 10.1094/MPMI.2004.17.5.447
M3 - Article
C2 - 15141948
AN - SCOPUS:1942504720
SN - 0894-0282
VL - 17
SP - 447
EP - 455
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 5
ER -