The HopPtoF Locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector

Libo Shan, Hye Sook Oh, Jianfu Chen, Ming Guo, Jianmin Zhou, James R. Alfano, Alan Collmer, Xu Jia, Xiaoyan Tang

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in P. syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF Pto and HopFPto, that are preceded by a single hrp box promoter. We present evidence here to show that ShcFPto and HopF Pto encode a type III chaperone and a cognate effector, respectively. ShcFPto interacts with and stabilizes the HopFPto protein in the bacterial cell. Translation of HopFPto starts at a rare initiation codon ATA that limits the synthesis of the HopFPto protein to a low level in bacterial cells.

Original languageEnglish (US)
Pages (from-to)447-455
Number of pages9
JournalMolecular Plant-Microbe Interactions
Volume17
Issue number5
DOIs
StatePublished - May 2004

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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