Abstract
The importance of discovering new chemical transformations and/or optimizing catalytic combinations has led to a flurry of activity in reaction screening. The in situ enzymatic screening (ISES) approach described here utilizes biological tools (enzymes/cofactors) to advance chemistry. The protocol interfaces an organic reaction layer with an adjacent aqueous layer containing reporting enzymes that act upon the organic reaction product, giving rise to a spectroscopic signal. ISES allows the experimentalist to rapidly glean information on the relative rates of a set of parallel organic/organometallic reactions under investigation, without the need to quench the reactions or draw aliquots. In certain cases, the real-time enzymatic readout also provides information on sense and magnitude of enantioselectivity and substrate specificity. This article contains protocols for single-well (relative rate) and double-well (relative rate/enantiomeric excess) ISES, in addition to a colorimetric ISES protocol and a miniaturized double-well procedure.
Original language | English (US) |
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Pages (from-to) | 285-305 |
Number of pages | 21 |
Journal | Current protocols in chemical biology |
Volume | 9 |
Issue number | 4 |
DOIs | |
State | Published - Dec 14 2017 |
Keywords
- UV/vis spectrophotometry
- catalysis
- enzymatic screening
- metal-ligand combinations
- reaction discovery
ASJC Scopus subject areas
- General Medicine