The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae)

T. Cherry, A. L. Szalanski, T. C. Todd, T. O. Powers

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

Belonolaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide sequence of a South Carolina Belonolaimus isolate. Amplified product size from individuals of each isolate was approximately 700 bp. All Midwestern isolates gave distinct restriction digestion patterns. Isolates identified morphologically as Belonolaimus longicaudatus from Florida, South Carolina, and Palm Springs, California, were identical for ITS1 restriction patterns. The correlation between ITS1 restriction patterns and the distribution of B. longicaudatus isolates suggest that the California isolate is a relatively recent introduction into the state.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalJournal of Nematology
Volume29
Issue number1
StatePublished - Mar 1997

Keywords

  • Belonolaimus
  • ITS
  • Molecular diagnostics
  • Ribosomal DNA
  • Sting nematode
  • Systematics
  • Taxonomy

ASJC Scopus subject areas

  • Agronomy and Crop Science

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