The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus

Sujata S. Chaudhari, Vinai C. Thomas, Marat R. Sadykov, Jeffrey L. Bose, Daniel J. Ahn, Matthew C. Zimmerman, Kenneth W. Bayles

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.

Original languageEnglish (US)
Pages (from-to)942-953
Number of pages12
JournalMolecular Microbiology
Volume101
Issue number6
DOIs
StatePublished - Sep 1 2016

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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