The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa

Ruiwen Wang; Michael G. Brattain

doi:10.1016/j.febslet.2007.05.082

The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa

Ruiwen Wang, Michael G. Brattain

Eppley Institute for Cancer Research

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

It has generally been believed that the diffusion limit set by the nuclear pore for protein is 60 kDa. We here studied the cellular localization of several artificial proteins and found that the diffusion limit set by the nuclear pore is not as small as previously thought. The results indicate that the maximal size of protein to diffuse through the nuclear pore complex could be quite larger than 60 kDa, thus greatly extending the diffusion limit that the nuclear pore can accommodate.

Original language	English (US)
Pages (from-to)	3164-3170
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2007.05.082
State	Published - Jul 10 2007

Keywords

Diffusion limit
Nuclear pore
Protein nuclear translocation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa",

abstract = "It has generally been believed that the diffusion limit set by the nuclear pore for protein is 60 kDa. We here studied the cellular localization of several artificial proteins and found that the diffusion limit set by the nuclear pore is not as small as previously thought. The results indicate that the maximal size of protein to diffuse through the nuclear pore complex could be quite larger than 60 kDa, thus greatly extending the diffusion limit that the nuclear pore can accommodate.",

keywords = "Diffusion limit, Nuclear pore, Protein nuclear translocation",

author = "Ruiwen Wang and Brattain, {Michael G.}",

note = "Funding Information: We are grateful to Dr. Lazebnik (Cold Spring Harbor Laboratory, NY) for the GFP5 construct, Dr. Cobb (University of Texas Southwestern Medical Center at Dallas, TX) for ERK2 cDNA, and Dr. Alessi (University of Dundee, UK) for Myc-PDK1 construct. This work was supported by National Institute of Health Grants CA034432 and CA054807 (to M.G.B).",

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T1 - The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa

AU - Wang, Ruiwen

AU - Brattain, Michael G.

N1 - Funding Information: We are grateful to Dr. Lazebnik (Cold Spring Harbor Laboratory, NY) for the GFP5 construct, Dr. Cobb (University of Texas Southwestern Medical Center at Dallas, TX) for ERK2 cDNA, and Dr. Alessi (University of Dundee, UK) for Myc-PDK1 construct. This work was supported by National Institute of Health Grants CA034432 and CA054807 (to M.G.B).

PY - 2007/7/10

Y1 - 2007/7/10

N2 - It has generally been believed that the diffusion limit set by the nuclear pore for protein is 60 kDa. We here studied the cellular localization of several artificial proteins and found that the diffusion limit set by the nuclear pore is not as small as previously thought. The results indicate that the maximal size of protein to diffuse through the nuclear pore complex could be quite larger than 60 kDa, thus greatly extending the diffusion limit that the nuclear pore can accommodate.

AB - It has generally been believed that the diffusion limit set by the nuclear pore for protein is 60 kDa. We here studied the cellular localization of several artificial proteins and found that the diffusion limit set by the nuclear pore is not as small as previously thought. The results indicate that the maximal size of protein to diffuse through the nuclear pore complex could be quite larger than 60 kDa, thus greatly extending the diffusion limit that the nuclear pore can accommodate.

KW - Diffusion limit

KW - Nuclear pore

KW - Protein nuclear translocation

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