TY - JOUR
T1 - The mechanism of microsomal hydroxylation of 7-methylbenz[a]-anthracene and 7,12-dimethylbenz[a]anthracene by oxygen-18 studies
AU - Grandjean, C.
AU - Cavalieri, E.
PY - 1974/12/11
Y1 - 1974/12/11
N2 - The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.
AB - The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.
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U2 - 10.1016/0006-291X(74)90242-3
DO - 10.1016/0006-291X(74)90242-3
M3 - Article
C2 - 4451566
AN - SCOPUS:0016313512
VL - 61
SP - 912
EP - 919
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -