The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 11 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology