The mechanism of microsomal hydroxylation of 7-methylbenz[a]-anthracene and 7,12-dimethylbenz[a]anthracene by oxygen-18 studies

C. Grandjean, E. Cavalieri

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.

Original languageEnglish (US)
Pages (from-to)912-919
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number3
DOIs
StatePublished - Dec 11 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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