TY - JOUR
T1 - The mechanism of microsomal hydroxylation of 7-methylbenz[a]-anthracene and 7,12-dimethylbenz[a]anthracene by oxygen-18 studies
AU - Grandjean, C.
AU - Cavalieri, E.
N1 - Funding Information:
Acknowledgment: Support of this research by Public Health Service contract 43-68-959 from the National Cancer Institute is gratefully acknowledged. We wish to express our thanks to Mrs. Laura Ryan for her valuable assistance, and to Dr. Phillip Issenberg and Mr. Steve Peratt for their mass spectral expertise.
PY - 1974/12/11
Y1 - 1974/12/11
N2 - The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.
AB - The carcinogenic 7-methylbenz[a]anthracene and 7,12-dimethylbenz[a]anthracene were converted by rat liver microsomes into the corresponding hydroxymethyl derivatives and other metabolic products. The 7-methylbenz[a]anthracene incubation was carried out in H218O, and no incorporation of oxygen-18 was found in the hydroxymethyl metabolite isolated and purified by high pressure liquid chromatography, and analyzed by mass spectrometry. When 7-methylbenz[a]anthracene or 7,12-dimethylbenz[a]anthracene was incubated with 18O2, isotope incorporation was observed in the corresponding hydroxymethyl derivatives, indicating that such hydroxylation is a true oxygenase reaction.
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U2 - 10.1016/0006-291X(74)90242-3
DO - 10.1016/0006-291X(74)90242-3
M3 - Article
C2 - 4451566
AN - SCOPUS:0016313512
VL - 61
SP - 912
EP - 919
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -