The metabolism of the pancreas carcinogen N-nitrosobis(2-oxopropyl)amine by hamster pancreas duct epithelial cell clones; evidence for different metabolic efficiencies and response to cytochrome P450 inducers

Carol Kolar, Terence Lawson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Context. We have isolated five stable clones from a primary culture of Syrian golden hamster pancreatic duct epithelial cells and have designated them as CK1 through CK5. Design. Here we describe the ability of two of these, CK1 and CK5, to metabolize the pancreas carcinogen N-nitrosobis(2-oxopropyl)amine. The metabolism was assessed as the production of mutated V79 cells in a CK cell/V79 co-culture set up. Results. At a dose of 0. 1 mM N-nitrosobis(2-oxopropyl)amine, the CK1 cells produced 82.3 ± 17.2 mutants/l06 survivors while the CK5 cells produced only 33.2 ± 10.8 mutants/106 survivors, both are mean ± SD (n = 8). Furthermore, both cell types responded differently to two inducers of cytochrome P450 activitN, namely Arochlor 1254 and EtOH. Arochlor 1254 treatment did not affect the metabolizing ability of CK1 cells while EtOH treatment resulted in a twofold increase in the mutation frequency. Arochlor and EtOH treatment inhibited the ability of CK5 cells to metabolize N-nitrosobis(2-oxopropyl)amine. Conclusions. These data show that the duct epithelium of the pancreas is a multi-cellular tissue and the different cell types within the epithelium have different abilities to metabolize xenobiotic chemicals.

Original languageEnglish (US)
Pages (from-to)13-18
Number of pages6
JournalJournal of the Pancreas
Volume1
Issue number1
StatePublished - May 2000

Keywords

  • Carcinogens
  • Epithelium
  • Metabolism
  • Mutagenesis
  • Nitrosamines
  • Pancreatic ducts

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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