Context. We have isolated five stable clones from a primary culture of Syrian golden hamster pancreatic duct epithelial cells and have designated them as CK1 through CK5. Design. Here we describe the ability of two of these, CK1 and CK5, to metabolize the pancreas carcinogen N-nitrosobis(2-oxopropyl)amine. The metabolism was assessed as the production of mutated V79 cells in a CK cell/V79 co-culture set up. Results. At a dose of 0. 1 mM N-nitrosobis(2-oxopropyl)amine, the CK1 cells produced 82.3 ± 17.2 mutants/l06 survivors while the CK5 cells produced only 33.2 ± 10.8 mutants/106 survivors, both are mean ± SD (n = 8). Furthermore, both cell types responded differently to two inducers of cytochrome P450 activitN, namely Arochlor 1254 and EtOH. Arochlor 1254 treatment did not affect the metabolizing ability of CK1 cells while EtOH treatment resulted in a twofold increase in the mutation frequency. Arochlor and EtOH treatment inhibited the ability of CK5 cells to metabolize N-nitrosobis(2-oxopropyl)amine. Conclusions. These data show that the duct epithelium of the pancreas is a multi-cellular tissue and the different cell types within the epithelium have different abilities to metabolize xenobiotic chemicals.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of the Pancreas|
|State||Published - May 2000|
- Pancreatic ducts
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism