TY - JOUR
T1 - The minimal transforming fragment of HSV-2 mtrIII can function as a complex promoter element
AU - Jones, Clinton
N1 - Funding Information:
This work was supported by grants from NIH (R29-CA47872, 2-507-RR05386) . I thank Drs . G . Chinchar, G . Gentry, and A . Razzaque for helpful discussions and a critical reading of the manuscript, L . Devine for the manuscript preparation, and D . Dale for artwork .
PY - 1989/4
Y1 - 1989/4
N2 - The minimal transforming fragment (486 TF) of HSV-2 mtrlll (0.567-0.570 map units) is composed of two distinct and non-overlapping promoter elements when linked to bacterial CAT genes. A 230-nucleotide fragment of 486 TF, SalI-Hpal, was active as a promoter element in primate cells but not rodent cells. A 173-nucleotide fragment, SmaIPstl, was active in both primate and rodent cells. The 486 TF did not compete for limiting cellular factors required to drive the CAT gene under control of the SV40 early promoter/enhancer. However, gel-retardation assays suggest that unique factors exist in cells transformed by HSV-2 which specifically recognized regions of 486 TF. These results are discussed with respect to HSV-2-mediated transformation.
AB - The minimal transforming fragment (486 TF) of HSV-2 mtrlll (0.567-0.570 map units) is composed of two distinct and non-overlapping promoter elements when linked to bacterial CAT genes. A 230-nucleotide fragment of 486 TF, SalI-Hpal, was active as a promoter element in primate cells but not rodent cells. A 173-nucleotide fragment, SmaIPstl, was active in both primate and rodent cells. The 486 TF did not compete for limiting cellular factors required to drive the CAT gene under control of the SV40 early promoter/enhancer. However, gel-retardation assays suggest that unique factors exist in cells transformed by HSV-2 which specifically recognized regions of 486 TF. These results are discussed with respect to HSV-2-mediated transformation.
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U2 - 10.1016/0042-6822(89)90160-8
DO - 10.1016/0042-6822(89)90160-8
M3 - Article
C2 - 2539696
AN - SCOPUS:0024550884
SN - 0042-6822
VL - 169
SP - 346
EP - 353
JO - Virology
JF - Virology
IS - 2
ER -