The nature of human immunodeficiency virus type 1 strand transfers

Hong Yu, Amanda E. Jetzt, Yacov Ron, Bradley D. Preston, Joseph P. Dougherty

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

The diploid nature of human immunodeficiency virus type 1 (HIV-1) suggests that recombination serves a central function in virus replication and evolution. A system was developed to examine HIV-1 strand transfers, including the obligatory DNA primer strand transfers as well as recombinational crossovers during reverse transcription. Sequence heterogeneity between different strains of HIV-1 was exploited for examining primer transfer events. Both intra- and intermolecular primer transfers were observed at similar frequencies during minus-strand DNA synthesis, whereas primer transfers during plus-strand DNA synthesis were primarily intramolecular. Sequence analysis of long terminal repeats from progeny proviruses also revealed a high rate of homologous recombination during minus-strand synthesis, corresponding to an overall rate of approximately three crossovers per HIV-1 genome per cycle of replication. These results imply that both viral genomic RNAs serve as templates during HIV-1 reverse transcription and that primer strand transfers and recombination may contribute substantially to the rapid genetic variation of HIV-1.

Original languageEnglish (US)
Pages (from-to)28384-28391
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number43
DOIs
StatePublished - Oct 23 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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