The neuropeptide Y/Peptide YY Y1 receptor is coupled to MAP kinase via PKC and Ras in CHO cells

Peter J. Mannon, John R. Raymond

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The neuropeptide Y/peptide YY (PYY) Y1 receptor subtype mediates proliferative responses. This report identifies effector molecules which mediate mitogen-activated protein kinase (MAPK) phosphorylation by Y1 receptor activation in transfected CHO cells. Pertussis toxin pretreatment abolishes this effect, indicating involvement of G(i) or G(o) proteins. Inhibition of protein kinase C (PKC) also blocks PYY-induced MAPK phosphorylation. Additionally in this cell model PYY causes an increase in GTP binding to Ras protein, and cotransfection of dominant negative constructs for Ras and Raf blocks PYY effects on MAPK. These data suggest a novel mechanism for Y1 receptor coupling to MAPK, which is at once pertussis toxin-sensitive as well as PKC- and Ras-dependent.

Original languageEnglish (US)
Pages (from-to)91-94
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number1
DOIs
StatePublished - May 8 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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