The pattern of gene expression in mouse Gr-1+ myeloid progenitor cells

Jianjun Chen, Donald A. Rowley, Terry Clark, Sanggyu Lee, Guolin Zhou, Christian Beck, Janet D. Rowley, San Ming Wang

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

To understand the pattern of gene expression in mouse myeloid progenitor cells, we carried out a genome-wide analysis of gene expression in mouse bone marrow Gr-1+ cells using SAGE and GLGI techniques. We identified 22,033 unique SAGE tags with quantitative information from 73,869 collected SAGE tags. Among these unique tags, 64% match known sequences, including many genes important for myeloid differentiation, and 36% have no matches to known sequences and are likely to represent novel genes. We compared the expression of mouse Gr-1+ and human CD15+ myeloid progenitor cells and showed that the pattern of gene expression of these two cell populations had some similarities. We also compared the expression of mouse Gr-1+ myeloid progenitor cells with that of mouse brain tissue and found a highly tissue-specific manner of gene expression in these two samples. Our data provide a basis for studying altered gene expression in myeloid disorders using mouse models.

Original languageEnglish (US)
Pages (from-to)149-162
Number of pages14
JournalGenomics
Volume77
Issue number3
DOIs
StatePublished - Aug 15 2001

Keywords

  • GLGI technique
  • Gene expression
  • Gene profile
  • Genome
  • Novel genes
  • SAGE technique

ASJC Scopus subject areas

  • Genetics

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